Team:Colombia Uniandes/How to parts

From 2013.igem.org

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| style="text-align: center;" |Primer FW
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| style="text-align: center;" |FW XbaI-GAL1
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| style="text-align: center;" |2,5 ul
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| style="text-align: center;" |CACCTCTAGAGGCTATATTGAAGTACGGATTAG
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| style="text-align: center;" |15
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|-  
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| style="text-align: center;" |Primer RV
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| style="text-align: center;" |FW Fusion GAL1-KOZAC-mCherry
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| style="text-align: center;" |2,5 ul
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| style="text-align: center;" |GGAAACTAGACCCGCCGCCACCATGGAGATGGTGAGCAAGGGCGAGG
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| style="text-align: center;" |17
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|-
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| style="text-align: center;" |DMSO
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| style="text-align: center;" |RV Fusion GAL1-KOZAC-mCherry
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| style="text-align: center;" |2,0 ul
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| style="text-align: center;" |CCTCGCCCTTGCTCACCATCTCCATGGTGGCGGCGGGTCTAGTTTCC
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| style="text-align: center;" |13
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|-
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| style="text-align: center;" |Master Mix
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| style="text-align: center;" |FW Fusion BAP2-KOZAC-GAL4
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| style="text-align: center;" |25 ul
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| style="text-align: center;" |CTCAAGTGATTTTAGAACCCGCCGCCACCATGGAGATGAAGCTACTGTCTTC
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| style="text-align: center;" |11
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| style="text-align: center;" |dH2O
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| style="text-align: center;" |RV Fusion BAP2-KOZAC-GAL4
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| style="text-align: center;" |15 ul
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| style="text-align: center;" |GAAGACAGTAGCTTCATCTCCATGGTGGCGGCGGGTTCTAAAATCACTTGAG
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| style="text-align: center;" |3
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|-
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| style="text-align: center;" |FW FUSION GAL4-VP16-1
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| style="text-align: center;" |CAGTTGACTGTATCTTCGAGGTCGACCATTCAGATCTCACGAGCCGCCCCCCCGACCG
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| style="text-align: center;" |1
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|-
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| style="text-align: center;" |RV FUSION GAL4-VP16-1
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| style="text-align: center;" |CGGTCGGGGGGGCGGCTCGTGAGATCTGAATGGTCGACCTCGAAGATACAGTCAACTG
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| style="text-align: center;" |5
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|-
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| style="text-align: center;" |FW FUSION VP6-GCR
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| style="text-align: center;" |CCTACGGCGCTCTGGATATGATCTCTCGAGCCATTCAGCAAGCCACTGCAGG
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| style="text-align: center;" |9
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|-
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| style="text-align: center;" |RV FUSION VP6-GCR
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| style="text-align: center;" |CCTGCAGTGGCTTGCTGAATGGCTCGAGAGATCATATCCAGAGCGCCGTAGG
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| style="text-align: center;" |6
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|-
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| style="text-align: center;" |FW Fusion GCR-yeastTerminador
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| style="text-align: center;" |GCTTCTGTTTCATCAAAAATGAAAAGTGAATATCCAAACGAGAATGTCAATGG
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| style="text-align: center;" |35
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|-
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| style="text-align: center;" |RV Fusion GCR-yeastTerminador
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| style="text-align: center;" |CCATTGACATTCTCGTTTGGATATTCACTTTTCATTTTTGATGAAACAGAAGC
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| style="text-align: center;" |34
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|-
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| style="text-align: center;" |FW Fusion mCherry-yeastTerminador
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| style="text-align: center;" |GGACGAGCTGTACAAGTAATAAAAAGTGAATATCCAAACGAGAATGTCAATGG
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| style="text-align: center;" |33
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|-
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| style="text-align: center;" |RV Fusion mCherry-yeastTerminador
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| style="text-align: center;" |CCATTGACATTCTCGTTTGGATATTCACTTTTTATTACTTGTACAGCTCGTCC
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| style="text-align: center;" |32
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|-
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| style="text-align: center;" |FW HoxN
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| style="text-align: center;" |ATGGAAGCACTGCCGAACGATT
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| style="text-align: center;" |42
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|-
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| style="text-align: center;" |RV HoxNconColamRfP
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| style="text-align: center;" |GTC TTC GGA GGA AGC CAT CTA TTC GAC GAA CCG
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| style="text-align: center;" |44
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|-
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| style="text-align: center;" |FW mRFPconColaHoxN
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| style="text-align: center;" |GTT CGT CGA ATA GAT GGC TTC CTC CGA AGA
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| style="text-align: center;" |41
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|-
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| style="text-align: center;" |RV mRFPconXba
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| style="text-align: center;" |ACT ATC TAG ATA TAA ACG CAG AAA GGC CCA CCC
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| style="text-align: center;" |38
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|-
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| style="text-align: center;" |FW Prcna
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| style="text-align: center;" |GCG GGG TTT TTT TTA CGG ATT GTA TGA GAC AT
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| style="text-align: center;" |43
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|-
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| style="text-align: center;" |RV Prcna
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| style="text-align: center;" |TCC ATT TTC TCC TCT TTG ATA ATA ATT CTT AGT AT
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| style="text-align: center;" |39
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|-
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| style="text-align: center;" |J23117+B0034+RcnR FW
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| style="text-align: center;" |GACAGCTAGCTCAGTCCTAGGGATTGTGCTAGCAAAGAGGAGAAAATGTCTCATACAATC
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| style="text-align: center;" |23
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|-
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| style="text-align: center;" |J23117+B0034+RcnR RV
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| style="text-align: center;" |GATTGTATGAGACATTTTCTCCTCTTTGCTAGCACAATCCCTAGGACTGAGCTAGCTGTC
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| style="text-align: center;" |21
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Revision as of 19:14, 25 September 2013

PCR reagents and amounts for one 50 ul reaction
Name Sequence Identification Number
FW XbaI-BAP2 CACCTCTAGAGAGCATTAATGCAAGTCGAGAAAGG 19
FW XbaI-GAL1 CACCTCTAGAGGCTATATTGAAGTACGGATTAG 15
FW Fusion GAL1-KOZAC-mCherry GGAAACTAGACCCGCCGCCACCATGGAGATGGTGAGCAAGGGCGAGG 17
RV Fusion GAL1-KOZAC-mCherry CCTCGCCCTTGCTCACCATCTCCATGGTGGCGGCGGGTCTAGTTTCC 13
FW Fusion BAP2-KOZAC-GAL4 CTCAAGTGATTTTAGAACCCGCCGCCACCATGGAGATGAAGCTACTGTCTTC 11
RV Fusion BAP2-KOZAC-GAL4 GAAGACAGTAGCTTCATCTCCATGGTGGCGGCGGGTTCTAAAATCACTTGAG 3
FW FUSION GAL4-VP16-1 CAGTTGACTGTATCTTCGAGGTCGACCATTCAGATCTCACGAGCCGCCCCCCCGACCG 1
RV FUSION GAL4-VP16-1 CGGTCGGGGGGGCGGCTCGTGAGATCTGAATGGTCGACCTCGAAGATACAGTCAACTG 5
FW FUSION VP6-GCR CCTACGGCGCTCTGGATATGATCTCTCGAGCCATTCAGCAAGCCACTGCAGG 9
RV FUSION VP6-GCR CCTGCAGTGGCTTGCTGAATGGCTCGAGAGATCATATCCAGAGCGCCGTAGG 6
FW Fusion GCR-yeastTerminador GCTTCTGTTTCATCAAAAATGAAAAGTGAATATCCAAACGAGAATGTCAATGG 35
RV Fusion GCR-yeastTerminador CCATTGACATTCTCGTTTGGATATTCACTTTTCATTTTTGATGAAACAGAAGC 34
FW Fusion mCherry-yeastTerminador GGACGAGCTGTACAAGTAATAAAAAGTGAATATCCAAACGAGAATGTCAATGG 33
RV Fusion mCherry-yeastTerminador CCATTGACATTCTCGTTTGGATATTCACTTTTTATTACTTGTACAGCTCGTCC 32
FW HoxN ATGGAAGCACTGCCGAACGATT 42
RV HoxNconColamRfP GTC TTC GGA GGA AGC CAT CTA TTC GAC GAA CCG 44
FW mRFPconColaHoxN GTT CGT CGA ATA GAT GGC TTC CTC CGA AGA 41
RV mRFPconXba ACT ATC TAG ATA TAA ACG CAG AAA GGC CCA CCC 38
FW Prcna GCG GGG TTT TTT TTA CGG ATT GTA TGA GAC AT 43
RV Prcna TCC ATT TTC TCC TCT TTG ATA ATA ATT CTT AGT AT 39
J23117+B0034+RcnR FW GACAGCTAGCTCAGTCCTAGGGATTGTGCTAGCAAAGAGGAGAAAATGTCTCATACAATC 23
J23117+B0034+RcnR RV GATTGTATGAGACATTTTCTCCTCTTTGCTAGCACAATCCCTAGGACTGAGCTAGCTGTC 21


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