Team:Marburg/Notebook:June
From 2013.igem.org
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<div class="notebooky-entry"> | <div class="notebooky-entry"> | ||
<h2 class="title"> | <h2 class="title"> | ||
- | <a name=" | + | <a name="02-07-2013">02.07.2013</a> |
</h2> | </h2> | ||
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</div> | </div> | ||
+ | <fieldset class="experiment digest"> | ||
+ | <legend><a name="dig">Digest</a></legend> | ||
+ | <div class="investigator"> | ||
+ | <span class="inv">Investigator:</span> | ||
+ | <span class="inv-names">Dominik</span> | ||
+ | </div> | ||
+ | <div class="aim"> | ||
+ | <span class="aim">Aim:</span> | ||
+ | <span class="aim-desc">Test digest of the plasmids pSB1A3-iBB10+iBB9, pSB1A3-iBB11+iBB9, pSB1A3-iBB12+iBB9 and pSB1A3-iBB13+iBB9 with <i>Eco</i>RI and <i>Pst</i>I</span> | ||
+ | </div> | ||
+ | <div class="exp-content"> | ||
+ | <p><ul class="digest"> | ||
+ | <li>1 µl Plasmid (pSB1A3-iBB10+iBB9, pSB1A3-iBB11+iBB9, pSB1A3-iBB12+iBB9 and pSB1A3-iBB13+iBB9)</li> | ||
+ | <li>0.3 µl EcoRI</li> | ||
+ | <li>0.3 µl PstI</li> | ||
+ | <li>1 µl CutSmart</li> | ||
+ | <li>7.4 µl ddH2O</li> | ||
+ | </ul> | ||
+ | <p>The samples were incubated for 1 h at 37° C.</p> | ||
+ | <table class="gel digest"> | ||
+ | <colgroup> | ||
+ | <col width="50%" /> | ||
+ | <col width="50%" /> | ||
+ | </colgroup> | ||
+ | <thead> | ||
+ | <tr> | ||
+ | <th colspan="2" class="title">Gel electrophoresis</th> | ||
+ | </tr> | ||
+ | </thead> | ||
+ | <tbody> | ||
+ | <tr> | ||
+ | <td> | ||
+ | <img src="beispiel.png" width="50%" alt="gel-electrophoresis-image" /> | ||
+ | </td> | ||
+ | <td> | ||
+ | <p> | ||
+ | <span class="gel-elc">Gel substances</span> | ||
+ | <ul class="gel-sub"> | ||
+ | <li>1% Agarose gel</li> | ||
+ | <li>10 µl RedSafe in 50 ml gel</li> | ||
+ | <li>x µl Hyper Ladder</li> | ||
+ | </ul> | ||
+ | </p> | ||
+ | <p> | ||
+ | <span class="exp">Expactations</span> | ||
+ | <ul class="exp"> | ||
+ | <li>Lane 1: 5300 kbp</li> | ||
+ | <li>Lane 2: 3300 kbp</li> | ||
+ | <li>Lane 3: 1300 kbp</li> | ||
+ | </ul> | ||
+ | </p> | ||
+ | <p>Worked as expected.</p> | ||
+ | </td> | ||
+ | </tr> | ||
+ | </tbody> | ||
+ | </table> | ||
+ | </div> | ||
+ | </fieldset> | ||
+ | </div> | ||
+ | <div class="notebooky-entry"> | ||
+ | <h2 class="title"> | ||
+ | <a name="23-07-2013">23.07.2013</a> | ||
+ | </h2> | ||
- | </ | + | <fieldset class="experiment digest"> |
+ | <legend><a name="dig">Digest</a></legend> | ||
+ | <div class="investigator"> | ||
+ | <span class="inv">Investigator:</span> | ||
+ | <span class="inv-names">Dominik</span> | ||
+ | </div> | ||
+ | <div class="aim"> | ||
+ | <span class="aim">Aim:</span> | ||
+ | <span class="aim-desc">Test digest of the plasmids pSB1C3-iBB4+iBB11+iBB9, pSB1C3-iBB4+iBB12+iBB9 with <i>Eco</i>RI and <i>Pst</i>I</span> | ||
+ | </div> | ||
+ | <div class="exp-content"> | ||
+ | <p><ul class="digest"> | ||
+ | <li>1 µl Plasmid (pSB1C3-iBB4+iBB11+iBB9 and pSB1C3-iBB4+iBB12+iBB9)</li> | ||
+ | <li>0.3 µl EcoRI</li> | ||
+ | <li>0.3 µl PstI</li> | ||
+ | <li>1 µl CutSmart</li> | ||
+ | <li>7.4 µl ddH2O</li> | ||
+ | </ul> | ||
+ | <p>The samples were incubated for 1 h at 37° C.</p> | ||
+ | <table class="gel digest"> | ||
+ | <colgroup> | ||
+ | <col width="50%" /> | ||
+ | <col width="50%" /> | ||
+ | </colgroup> | ||
+ | <thead> | ||
+ | <tr> | ||
+ | <th colspan="2" class="title">Gel electrophoresis</th> | ||
+ | </tr> | ||
+ | </thead> | ||
+ | <tbody> | ||
+ | <tr> | ||
+ | <td> | ||
+ | <img src="beispiel.png" width="50%" alt="gel-electrophoresis-image" /> | ||
+ | </td> | ||
+ | <td> | ||
+ | <p> | ||
+ | <span class="gel-elc">Gel substances</span> | ||
+ | <ul class="gel-sub"> | ||
+ | <li>1% Agarose gel</li> | ||
+ | <li>10 µl RedSafe in 50 ml gel</li> | ||
+ | <li>x µl Hyper Ladder</li> | ||
+ | </ul> | ||
+ | </p> | ||
+ | <p> | ||
+ | <span class="exp">Expactations</span> | ||
+ | <ul class="exp"> | ||
+ | <li>Lane 1: 5300 kbp</li> | ||
+ | <li>Lane 2: 3300 kbp</li> | ||
+ | <li>Lane 3: 1300 kbp</li> | ||
+ | </ul> | ||
+ | </p> | ||
+ | <p>Worked as expected.</p> | ||
+ | </td> | ||
+ | </tr> | ||
+ | </tbody> | ||
+ | </table> | ||
+ | </div> | ||
+ | </fieldset> | ||
+ | </div> | ||
+ | <div class="notebooky-entry"> | ||
+ | <h2 class="title"> | ||
+ | <a name="24-07-2013">24.07.2013</a> | ||
+ | </h2> | ||
+ | |||
+ | <fieldset class="experiment inoculation"> | ||
+ | <legend><a name="ino">Inoculation</a></legend> | ||
+ | <div class="investigator"> | ||
+ | <span class="inv">Investigator:</span> | ||
+ | <span class="inv-names">Dominik</span> | ||
+ | </div> | ||
+ | <div class="aim"> | ||
+ | <span class="aim">Aim:</span> | ||
+ | <span class="aim-desc">inoculation of colonies for plasmid preparation</span> | ||
+ | </div> | ||
+ | <div class="exp-content"> | ||
+ | <p>4 colonies of pSB1C3-iBB4+iBB10+iBB9 and 4 colonies of pSB1C3-iBB4+iBB13+iBB9 were inoculated in 5 ml LB medium containing chloramphenicol.</p> | ||
+ | </div> | ||
+ | </fieldset> | ||
+ | </div> | ||
+ | |||
+ | </html> | ||
{{:Team:Marburg/Template:ContentEnd}} | {{:Team:Marburg/Template:ContentEnd}} | ||
{{:Team:Marburg/Template:Footer}} | {{:Team:Marburg/Template:Footer}} |
Revision as of 11:58, 26 September 2013