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Team:Paris Saclay/Notebook/July/8
From 2013.igem.org
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* Well 1 : 6µL DNA Ladder | * Well 1 : 6µL DNA Ladder | ||
Revision as of 22:31, 28 September 2013
Contents |
Notebook : July 8
Lab work
A - Aerobic/Anaerobic regulation system
Objective : Bba_K1155003, Bba_K1155007
1 - Digestion of Bba_K592009, Bba_I732017 by NotI, XhoI, EcoRI, EcoRI/PstI
Abou, Anaïs, Sheng, Zhou
Used quantities :
- NotI :
- Bba_K592009 : 2µL
- Buffer orange : 2µL
- NotI : 0.5µL
- H2O : 15.5µL
- XhoI :
- Bba_K592009 : 2µL
- Buffer red : 2µL
- XhoI : 0.5µL
- H2O : 15.5µL
- EcoRI :
- Bba_K592009 : 2µL
- Buffer orange : 2µL
- EcoRI : 0.5µL
- H2O : 15.5µL
- EcoRI/PstI :
- Bba_I732017 : 2µL
- Buffer orange : 2µL
- EcoRI : 0.5µL
- PstI : 0.5µL
- H2O : 15µL
We let our digestion 1h30 at 37°C.
2 - Electrophoresis of the digestion of Bba_K592009, Bba_I732017 by NotI, XhoI, EcoRI, EcoRI/PstI
Abdou, Anaïs, Sheng, Zhou
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Expected sizes :
- Bba_K592009 digested by NotI : 2046bp + 693bp
- Bba_K592009 digested by XhoI : 1842bp + 892bp
- Bba_I732017 : ...
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We obtain fragments at the right size. Our extraction was good. |
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