Team:UANL Mty-Mexico/Notebook

From 2013.igem.org

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<p><b>August 10th, 2013</b></p>
<p><b>August 10th, 2013</b></p>
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<p>20 tubes with mCherry were put in the shaker with 2 controls with CDS (with oxygen everyone).
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<p>20 tubes with mCherry were put in the shaker with 2 controls with CDS (with oxygen everyone). </p>
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</p>
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<p>Temperature: 37ᵒ</p>
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<p>Revolutions: 900 rpm</p>
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<p>Start: 11:35 am ->  9:00 am (Time 21: 35 hours)</p>
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<p>The tubes show mCherry expression, some more intense than others.The experiment was repeated at 30°C OVERNIGHT with a
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Volume of 500ul at 30°C with tubes of 1.5ml. They were left at 4°C meanwhile they were incubated.
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</p>
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<table class="table table-striped">
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<tr>
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<th><b>Set</b></th>
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<th><b>Temperature</b></th>
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<th><b>Velocity</b></th>
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<th><b>Time</b></th>
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<th><b>Live</b></th>
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</tr>
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<tr>
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<td> Black Set</td>
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<td>42ºC</td>
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<td>900rpm</td>
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<td>15 hours</td>
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<td> :( </td>
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</tr>
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<tr>
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<td>Blue Set</td>
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<td> 37ºC </td>
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<td>900rpm</td>
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<td>21 hours</td>
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<td> :) </td>
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</tr>
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<tr>
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<td>Red Set</td>
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<td> 32ºC</td>
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<td>900rpm</td>
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<td></td>
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<td>:)</td>
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</tr>
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</table>
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Revision as of 20:06, 27 September 2013

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Notebook

Reference Data

Part Name Part Short name In pSB1A3 (bp) In pSB1C3 (bp) In PUC57 (bp) Part size (bp)
pLambdaCI + TetR TetR 2925 2802 3478 732
pLac + GFP GFP 3162 3034 3715 964
pCons LacI LacI 3483 3352 4036 1,282
pTetR+mCherry mCherry 3107 2957 3660 887
BBa_K1140005 3-E1 2811 --- --- 935

This table works as a reference of the parts that were used in the laboratory and in the diary. We have a short name for each part so for example, if a diary entry says "TetR" we mean the complete part "pLambdaCI + TetR". Another important aspect is that we were working with the plasmids pSB1C3, pSB1A3 and PUC57 so here there is the information about the length of the part and the length in each plasmid.

August 7th, 2013

This day we did Minipreparation of DNA. The parts that were obtained were the following: pCos R Lac1, pLac R GFP, pTetR R mCherry, pTetR, 3-1E

August 8th, 2013

Test of mCherry with Thermo-mixer-Cualitative experiment

20 Colonies of the dish with pTet-R-mCherry were planted in 0.5 mL of Agar with Kanamycin in order to observe the red color after several hours at 42ᵒ C

Start End Hours
37ºC 2:50 pm 7:02 pm 4 hr 12 min
42ºC 7:05 pm 10:35 am 15 hr 40 min

Most tubes shown development seeing them on the light.

August 10th, 2013

20 tubes with mCherry were put in the shaker with 2 controls with CDS (with oxygen everyone).

Temperature: 37ᵒ

Revolutions: 900 rpm

Start: 11:35 am -> 9:00 am (Time 21: 35 hours)

The tubes show mCherry expression, some more intense than others.The experiment was repeated at 30°C OVERNIGHT with a Volume of 500ul at 30°C with tubes of 1.5ml. They were left at 4°C meanwhile they were incubated.

Set Temperature Velocity Time Live
Black Set 42ºC 900rpm 15 hours :(
Blue Set 37ºC 900rpm 21 hours :)
Red Set 32ºC 900rpm :)

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