Team:SJTU-BioX-Shanghai/Modeling/Report gene prediction
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- | We choose constitutive RFP as our preliminary report gene. Cph8 has been choosen as the light control system combined with dCas9 system.When we | + | We choose constitutive RFP as our preliminary report gene. Cph8 has been choosen as the light control system combined with dCas9 system. When we raise the red light intensity, Cph8 will inhibit the sgRNA transcription. With the decrease of the combination between sgRNA and dCas9, the expression of RFP will be up-regulated. So we expect that the red light intensity should be positive correlation with the RFP expression. |
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Revision as of 02:23, 28 September 2013