Team:Colombia Uniandes/Parts

From 2013.igem.org

(Difference between revisions)
(Glucocorticoid sensor)
(Glucocorticoid sensor)
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Also, the fluorescence assays for the E*GF parts
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[[File:Response1hr.jpg|400px|thumb|left|''' Unsaturated curve where we see how the fluorescence increases after the addition of dexamethasone, a glucocorticoid''']]
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[[File:Response3hr.jpg|400px|thumb|right|''' Saturated curve for fluorescence. We can see the different strengths depending on the number of UAS boxes''']]

Revision as of 02:25, 28 September 2013

Parts

Glucocorticoid sensor

Here are the parts sent to the registry:

PartsGC.jpg

You can see the confirmation gels of the parts below:



PCR confirmation: E1GF-E1GF(2)-E2GF-E2GF(2)-WM-E3GF-E3GF(2)-E4GF-E4GF(2)
Co-transformation: WM-E1GF2-E2GF-E3GF1-E3GF2-E4GF-WM- E3GF3-E4GF2-E2GF2


Also, the fluorescence assays for the E*GF parts

Unsaturated curve where we see how the fluorescence increases after the addition of dexamethasone, a glucocorticoid
Saturated curve for fluorescence. We can see the different strengths depending on the number of UAS boxes


Nickel Removal System

Here are the parts sent to the registry:

PartsNi.jpg
Prcna-HoxN and HoxN-RFP