Team:Colombia Uniandes/Parts

From 2013.igem.org

(Difference between revisions)
(→Glucocorticoid sensor)
(→Glucocorticoid sensor)
Line 22: Line 22:
[[File:partsGC.jpg|700px|center|]]
[[File:partsGC.jpg|700px|center|]]
 +
{| border="1" align="center"
 +
|+'''Name table'''
 +
| style="text-align: center;" |Part Name
 +
| style="text-align: center;" |Registry Number
 +
|-
 +
| style="text-align: center;" |E1
 +
| style="text-align: center;" |BBa_K1144001
 +
|-
 +
| style="text-align: center;" |E2
 +
| style="text-align: center;" |BBa_K1144002
 +
|-
 +
| style="text-align: center;" |E3
 +
| style="text-align: center;" |BBa_K1144003
 +
|-
 +
| style="text-align: center;" |E4
 +
| style="text-align: center;" |BBa_K1144004
 +
|-
 +
| style="text-align: center;" |E1GF
 +
| style="text-align: center;" |BBa_K1144005
 +
|-
 +
| style="text-align: center;" |E2GF
 +
| style="text-align: center;" |BBa_K1144006
 +
|-
 +
| style="text-align: center;" |E3GF
 +
| style="text-align: center;" |BBa_K1144007
 +
|-
 +
| style="text-align: center;" |E4GF
 +
| style="text-align: center;" |BBa_K1144008
 +
 +
|-
 +
|}
You can see the confirmation gels of the parts below:  
You can see the confirmation gels of the parts below:  

Revision as of 03:01, 28 September 2013

Parts

Glucocorticoid sensor

Glucocorticoid sensor

Here are the parts sent to the registry:

PartsGC.jpg
Name table
Part Name Registry Number
E1 BBa_K1144001
E2 BBa_K1144002
E3 BBa_K1144003
E4 BBa_K1144004
E1GF BBa_K1144005
E2GF BBa_K1144006
E3GF BBa_K1144007
E4GF BBa_K1144008

You can see the confirmation gels of the parts below:




PCR confirmation: E1GF-E1GF(2)-E2GF-E2GF(2)-WM-E3GF-E3GF(2)-E4GF-E4GF(2)
Co-transformation: WM-E1GF2-E2GF-E3GF1-E3GF2-E4GF-WM- E3GF3-E4GF2-E2GF2
PCR confirmation of the parts cloned in the pSB1C3 backbone using the specifically designed primers 15 and 31 (see primers)


Also, the fluorescence assays for the E*GF parts

Unsaturated curve where we see how the mCherry reporter appears after the addition of 10 uM dexamethasone, a glucocorticoid. Emmision intensity was measured at 607nm after excitation at 586nm


Saturated curve for fluorescence. After three hours of the addition of 10 uM dexamethasone the reporter (mCherry) reaches its highest signal. We can see the different strengths depending on the number of UAS boxes. Emmision intensity was measured at 607nm after excitation at 586nm


Cells with the E2GF part (BBa_K1144006) showing their fluorescent reporter, mCherry, after induction with 10 uM dexamethasone! The filter used was TRITC
Cells with the E4GF part (BBa_K1144008) showing their fluorescent reporter, mCherry, after induction with 10 uM dexamethasone! The filter used was TRITC 2


Nickel Removal System

Nickel Removal System

Here are the parts sent to the registry:

PartsNi.jpg
Prcna-HoxN and HoxN-RFP


Retrieved from "http://2013.igem.org/Team:Colombia_Uniandes/Parts"