Team:Marburg/Notebook:April

From 2013.igem.org

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Notebook: April
Notebook: April
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<div class="notebooky-entry">
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<h2 class="title">
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<a name="26-04-2013">26.04.2013</a>
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</h2>
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<!-- Sequencing -->
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<fieldset class="experiment sequencing">
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    <legend>Sequencing</legend>
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    <div class="investigator">
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<span class="inv">Investigator:</span>
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<span class="inv-names">Franzi</span>
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</div>
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    <div class="aim">
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<span class="aim">Aim:</span>
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<span class="aim-desc">Examination of sequencing results (23.4.13).</span>
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</div>
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<div class="exp-content">
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<p>1C3 iBB1 K1 + K2 correct</p>
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<p>1C3 iBB4 K1 + K2 correct</p>
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<p>1C3 iBB7 K3 + K4 correct</p>
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<p>1C3 iBB5 K1 + K2 Mutagenesis of XhoI-Site failed, only necessary for iGEM-standard 10 &rarr; unnecessary, rest correct</p>
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<p>1C3 iBB3 K1 + K2 same point-Mutation &rarr; template?</p>
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<p>1C3 iBB6 K1 + K2 same point-mutation &rarr; template?</p>
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</div>
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</fieldset>
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<!-- Sequencing -->
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<fieldset class="experiment sequencing">
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    <legend>Sequencing</legend>
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    <div class="investigator">
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<span class="inv">Investigator:</span>
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<span class="inv-names">Franzi</span>
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</div>
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    <div class="aim">
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<span class="aim">Aim:</span>
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<span class="aim-desc">Sequencing of pPhaNR.</span>
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</div>
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<div class="exp-content">
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<p>Original pPhaNR was sent to sequencing using primers imR25/26 for iBB3 and iBB6.</p>
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</div>
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</fieldset>
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<!-- Miniprep -->
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<fieldset class="experiment miniprep">
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    <legend><a name="min">Miniprep</a></legend>
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    <div class="investigator">
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<span class="inv">Investigator:</span>
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<span class="inv-names">Franzi</span>
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</div>
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    <div class="aim">
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<span class="aim">Aim:</span>
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<span class="aim-desc">TBA</span>
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</div>
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<div class="exp-content">
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<p>PSB1C3 iBB8 K1 and pSB1C3 iBB2 K1-4 were isolated via Miniprep, eluted in 40 l Elutionbuffer.
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</p>
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</div>
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</fieldset>
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<!-- Digest -->
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<fieldset class="experiment digest">
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    <legend><a name="dig">Digest</a></legend>
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    <div class="investigator">
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<span class="inv">Investigator:</span>
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<span class="inv-names">Franzi</span>
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</div>
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    <div class="aim">
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<span class="aim">Aim:</span>
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<span class="aim-desc">TBA</span>
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</div>
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<div class="exp-content">
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<ul class="digest">
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<li>1 µl DNA template</li>
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<li>0,5 µl <i>EcoR</i>I</li>
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<li>0,5 µl <i>Pst</i></li>
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<li>1 µl Cut Smart buffer</li>
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<li>6x loading buffer</li>
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<li>7 µl H2O</li>
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</ul>
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<p>The samples were incubated for 1 h at 37° C.</p>
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<table class="gel digest">
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<colgroup>
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<col width="50%" />
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<col width="50%" />
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</colgroup>
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<thead>
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<tr>
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<th colspan="2" class="title">Gel electrophoresis</th>
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</tr>
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</thead>
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<tbody>
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<tr>
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<td>
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<img src="beispiel.png" width="50%" alt="gel-electrophoresis-image" />
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</td>
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<td>
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<p>
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<span class="gel-elc">Gel substances</span>
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<ul class="gel-sub">
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<li>1% Agarose gel</li>
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<li>10 µl RedSafe per 50 ml gel</li>
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<li>6 µl GeneRuler&trade; 1kb plus DNA Ladder (Thermo Scientific)</li>
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</ul>
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</p>
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<p>IBB8 negative, iBB2 all positive.</p>
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</td>
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</tr>
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</tbody>
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</table>
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</div>
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</fieldset>
 +
 +
<!-- Sequencing -->
 +
<fieldset class="experiment sequencing">
 +
    <legend>Sequencing</legend>
 +
    <div class="investigator">
 +
<span class="inv">Investigator:</span>
 +
<span class="inv-names">Franzi</span>
 +
</div>
 +
    <div class="aim">
 +
<span class="aim">Aim:</span>
 +
<span class="aim-desc">Sequencing of pSB1C3 iBB2.</span>
 +
</div>
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<div class="exp-content">
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<p>IBB2 K2 + K4 sequenced with primer VF2_fwd and VR_rev.</p>
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</div>
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</fieldset>
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</div>
</html>
</html>
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{{:Team:Marburg/Template:Footer}}

Revision as of 16:44, 30 September 2013

Notebook: April

26.04.2013

Sequencing
Investigator: Franzi
Aim: Examination of sequencing results (23.4.13).

1C3 iBB1 K1 + K2 correct

1C3 iBB4 K1 + K2 correct

1C3 iBB7 K3 + K4 correct

1C3 iBB5 K1 + K2 Mutagenesis of XhoI-Site failed, only necessary for iGEM-standard 10 → unnecessary, rest correct

1C3 iBB3 K1 + K2 same point-Mutation → template?

1C3 iBB6 K1 + K2 same point-mutation → template?

Sequencing
Investigator: Franzi
Aim: Sequencing of pPhaNR.

Original pPhaNR was sent to sequencing using primers imR25/26 for iBB3 and iBB6.

Miniprep
Investigator: Franzi
Aim: TBA

PSB1C3 iBB8 K1 and pSB1C3 iBB2 K1-4 were isolated via Miniprep, eluted in 40 l Elutionbuffer.

Digest
Investigator: Franzi
Aim: TBA
  • 1 µl DNA template
  • 0,5 µl EcoRI
  • 0,5 µl Pst
  • 1 µl Cut Smart buffer
  • 6x loading buffer
  • 7 µl H2O

The samples were incubated for 1 h at 37° C.

Gel electrophoresis
gel-electrophoresis-image

Gel substances

  • 1% Agarose gel
  • 10 µl RedSafe per 50 ml gel
  • 6 µl GeneRuler™ 1kb plus DNA Ladder (Thermo Scientific)

IBB8 negative, iBB2 all positive.
Sequencing
Investigator: Franzi
Aim: Sequencing of pSB1C3 iBB2.

IBB2 K2 + K4 sequenced with primer VF2_fwd and VR_rev.

Retrieved from "http://2013.igem.org/Team:Marburg/Notebook:April"