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Team:Paris Saclay/Notebook/June/26
From 2013.igem.org
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=='''Lab work'''== | =='''Lab work'''== | ||
| - | - Design | + | - Design the oligonucleotide for the amplification of Pbphr1 (intergenic sequence upstream) for ''Pseudomonas |
| - | pseudoalcaligenes PbphD and KF707 (intergenic sequence upstream) for Burkholderia xenovorans LB400 | + | pseudoalcaligenes'' PbphD and KF707 (intergenic sequence upstream) for ''Burkholderia xenovorans'' LB400 |
- 500 ml Buffers (TB: the Transformation Buffer) prepared to make super competent bacteria, stored at 4°C | - 500 ml Buffers (TB: the Transformation Buffer) prepared to make super competent bacteria, stored at 4°C | ||
| - | - 250 ml of culture of E.Coli DH5αZ1 in LB prepared for overnight culture, with an initial | + | - 250 ml of culture of ''E.Coli'' DH5αZ1 in LB prepared for overnight culture, with an initial OD (Optical Density) of 0.04 at 17h30min |
| - | - Protocol of extraction of the genomic DNA from bacteria (E.Coli DH5α) written | + | - Protocol of extraction of the genomic DNA from bacteria (''E.Coli'' DH5α) written |
- Documentation about the fnr activator operator sequences in iGEM registry | - Documentation about the fnr activator operator sequences in iGEM registry | ||
Latest revision as of 00:29, 5 October 2013
Notebook : June 26
Lab work
- Design the oligonucleotide for the amplification of Pbphr1 (intergenic sequence upstream) for Pseudomonas pseudoalcaligenes PbphD and KF707 (intergenic sequence upstream) for Burkholderia xenovorans LB400
- 500 ml Buffers (TB: the Transformation Buffer) prepared to make super competent bacteria, stored at 4°C
- 250 ml of culture of E.Coli DH5αZ1 in LB prepared for overnight culture, with an initial OD (Optical Density) of 0.04 at 17h30min
- Protocol of extraction of the genomic DNA from bacteria (E.Coli DH5α) written
- Documentation about the fnr activator operator sequences in iGEM registry
Human practices
- Team meeting and discussion with Munich team (Skype)
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