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We heterologously expressed the porin protein OprF from Pseudomonas fluorescens into Escherichia coli. This leads to dramatically increased membrane permeability and a much higher current output in comparison to its parental strain (E. coli KRX) caused by improved electron shuttle-mediated extracellular electron transfer. The heterologous expression of outer membrane porin OprF from Pseudomonas fluorescens in Escherichia coli is a great genetic strategy to improve electricity generation by microorganisms.

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	==Porins==		==Porins==

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		+	*We heterologously expressed the porin protein OprF from ''Pseudomonas fluorescens'' into ''Escherichia coli''. This leads to dramatically increased membrane permeability and a much higher current output in comparison to its parental strain (''E. coli'' KRX) caused by improved electron shuttle-mediated extracellular electron transfer. The heterologous expression of outer membrane porin OprF from ''Pseudomonas fluorescens'' in ''Escherichia coli'' is a great genetic strategy to improve electricity generation by microorganisms.