Team:Marburg/Notebook:Primer list

From 2013.igem.org

(Difference between revisions)
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{{:Team:Marburg/Template:ContentStartNav}}<html>
{{:Team:Marburg/Template:ContentStartNav}}<html>
<table id="primer_list">
<table id="primer_list">
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  <col width=110>
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  <col width=178>
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  <tr>
   <td height=21 class=xl72 width=44 style='height:15.75pt;width:33pt'>No</td>
   <td height=21 class=xl72 width=44 style='height:15.75pt;width:33pt'>No</td>
Line 15: Line 13:
   <td class=xl73 width=503 style='border-left:none;width:377pt'>Sequence</td>
   <td class=xl73 width=503 style='border-left:none;width:377pt'>Sequence</td>
   <td class=xl74 width=229 style='border-left:none;width:172pt'>Comment</td>
   <td class=xl74 width=229 style='border-left:none;width:172pt'>Comment</td>
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  <td class=xl68 width=122 style='width:92pt'></td>
 
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  </tr>
  </tr>
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Line 23: Line 19:
   <td class=xl69 style='border-top:none;border-left:none'>GTTTCTTCGAATTCGCGGCCGCTTCTAGAATGGCCAAGTTGACCAGTGCCGTTC</td>
   <td class=xl69 style='border-top:none;border-left:none'>GTTTCTTCGAATTCGCGGCCGCTTCTAGAATGGCCAAGTTGACCAGTGCCGTTC</td>
   <td class=xl76 style='border-top:none;border-left:none'>&nbsp;</td>
   <td class=xl76 style='border-top:none;border-left:none'>&nbsp;</td>
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  <td class=xl68></td>
 
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  </tr>
  </tr>
  <tr>
  <tr>
Line 31: Line 25:
   <td class=xl69 style='border-top:none;border-left:none'>GTTTCTTCCTGCAGCGGCCGCTACTAGTTCAGTCCTGCTCCTCGGCCACGAAG</td>
   <td class=xl69 style='border-top:none;border-left:none'>GTTTCTTCCTGCAGCGGCCGCTACTAGTTCAGTCCTGCTCCTCGGCCACGAAG</td>
   <td class=xl76 style='border-top:none;border-left:none'>&nbsp;</td>
   <td class=xl76 style='border-top:none;border-left:none'>&nbsp;</td>
-
  <td class=xl68></td>
 
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  <td class=xl68></td>
 
  </tr>
  </tr>
  <tr>
  <tr>
Line 39: Line 31:
   <td class=xl69 style='border-top:none;border-left:none'>GTTTCTTCGAATTCGCGGCCGCTTCTAGAATGGAGAAAAAAATCACTGGATATA</td>
   <td class=xl69 style='border-top:none;border-left:none'>GTTTCTTCGAATTCGCGGCCGCTTCTAGAATGGAGAAAAAAATCACTGGATATA</td>
   <td class=xl76 style='border-top:none;border-left:none'>&nbsp;</td>
   <td class=xl76 style='border-top:none;border-left:none'>&nbsp;</td>
-
  <td class=xl68></td>
 
-
  <td class=xl68></td>
 
  </tr>
  </tr>
  <tr>
  <tr>
Line 47: Line 37:
   <td class=xl69 style='border-top:none;border-left:none'>GTTTCTTCCTGCAGCGGCCGCTACTAGTTTACGCCCCGCCCTGCCACTCATCGC</td>
   <td class=xl69 style='border-top:none;border-left:none'>GTTTCTTCCTGCAGCGGCCGCTACTAGTTTACGCCCCGCCCTGCCACTCATCGC</td>
   <td class=xl76 style='border-top:none;border-left:none'>&nbsp;</td>
   <td class=xl76 style='border-top:none;border-left:none'>&nbsp;</td>
-
  <td class=xl68></td>
 
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  <td class=xl68></td>
 
  </tr>
  </tr>
  <tr>
  <tr>
Line 55: Line 43:
   <td class=xl69 style='border-top:none;border-left:none'>GTTTCTTCGAATTCGCGGCCGCTTCTAGAATCCGGAGAGATTCAAAATTCAATC</td>
   <td class=xl69 style='border-top:none;border-left:none'>GTTTCTTCGAATTCGCGGCCGCTTCTAGAATCCGGAGAGATTCAAAATTCAATC</td>
   <td class=xl76 style='border-top:none;border-left:none'>&nbsp;</td>
   <td class=xl76 style='border-top:none;border-left:none'>&nbsp;</td>
-
  <td class=xl68></td>
 
-
  <td class=xl68></td>
 
  </tr>
  </tr>
  <tr>
  <tr>
Line 63: Line 49:
   <td class=xl69 style='border-top:none;border-left:none'>GTTTCTTCCTGCAGCGGCCGCTACTAGTCTTGACATCTGGCAACCGTGAAATA</td>
   <td class=xl69 style='border-top:none;border-left:none'>GTTTCTTCCTGCAGCGGCCGCTACTAGTCTTGACATCTGGCAACCGTGAAATA</td>
   <td class=xl76 style='border-top:none;border-left:none'>&nbsp;</td>
   <td class=xl76 style='border-top:none;border-left:none'>&nbsp;</td>
-
  <td class=xl68></td>
 
-
  <td class=xl68></td>
 
  </tr>
  </tr>
  <tr>
  <tr>
Line 71: Line 55:
   <td class=xl69 style='border-top:none;border-left:none'>GTTTCTTCGAATTCGCGGCCGCTTCTAGACATATGCGGAAGTGACTGTAAACGA</td>
   <td class=xl69 style='border-top:none;border-left:none'>GTTTCTTCGAATTCGCGGCCGCTTCTAGACATATGCGGAAGTGACTGTAAACGA</td>
   <td class=xl76 style='border-top:none;border-left:none'>&nbsp;</td>
   <td class=xl76 style='border-top:none;border-left:none'>&nbsp;</td>
-
  <td class=xl68></td>
 
-
  <td class=xl68></td>
 
  </tr>
  </tr>
  <tr>
  <tr>
Line 79: Line 61:
   <td class=xl69 style='border-top:none;border-left:none'>GTTTCTTCCTGCAGCGGCCGCTACTAGTCGTTCGCACAAGTGGTGACTTTGAA</td>
   <td class=xl69 style='border-top:none;border-left:none'>GTTTCTTCCTGCAGCGGCCGCTACTAGTCGTTCGCACAAGTGGTGACTTTGAA</td>
   <td class=xl76 style='border-top:none;border-left:none'>&nbsp;</td>
   <td class=xl76 style='border-top:none;border-left:none'>&nbsp;</td>
-
  <td class=xl68></td>
 
-
  <td class=xl68></td>
 
  </tr>
  </tr>
  <tr>
  <tr>
Line 87: Line 67:
   <td class=xl69 style='border-top:none;border-left:none'>GTTTCTTCGAATTCGCGGCCGCTTCTAGACCTTCCTTAAAAATTTAATTTTCAT</td>
   <td class=xl69 style='border-top:none;border-left:none'>GTTTCTTCGAATTCGCGGCCGCTTCTAGACCTTCCTTAAAAATTTAATTTTCAT</td>
   <td class=xl76 style='border-top:none;border-left:none'>&nbsp;</td>
   <td class=xl76 style='border-top:none;border-left:none'>&nbsp;</td>
-
  <td class=xl68></td>
 
-
  <td class=xl68></td>
 
  </tr>
  </tr>
  <tr>
  <tr>
Line 96: Line 74:
   TCATCCTGTG CCTT</td>
   TCATCCTGTG CCTT</td>
   <td class=xl76 style='border-top:none;border-left:none'>&nbsp;</td>
   <td class=xl76 style='border-top:none;border-left:none'>&nbsp;</td>
-
  <td class=xl68></td>
 
-
  <td class=xl68></td>
 
  </tr>
  </tr>
  <tr>
  <tr>
Line 104: Line 80:
   <td class=xl70 style='border-top:none;border-left:none'>GTTTCTTCGAATTCGCGGCCGCTTCTAGACAATTTACTTTAAGACTAGC</td>
   <td class=xl70 style='border-top:none;border-left:none'>GTTTCTTCGAATTCGCGGCCGCTTCTAGACAATTTACTTTAAGACTAGC</td>
   <td class=xl76 style='border-top:none;border-left:none'>&nbsp;</td>
   <td class=xl76 style='border-top:none;border-left:none'>&nbsp;</td>
-
  <td class=xl68></td>
 
-
  <td class=xl68></td>
 
  </tr>
  </tr>
  <tr>
  <tr>
Line 112: Line 86:
   <td class=xl70 style='border-top:none;border-left:none'>GTTTCTTCCTGCAGCGGCCGCTACTAGTTCCGGATGCGTTCATTTTAG</td>
   <td class=xl70 style='border-top:none;border-left:none'>GTTTCTTCCTGCAGCGGCCGCTACTAGTTCCGGATGCGTTCATTTTAG</td>
   <td class=xl76 style='border-top:none;border-left:none'>&nbsp;</td>
   <td class=xl76 style='border-top:none;border-left:none'>&nbsp;</td>
-
  <td class=xl68></td>
 
-
  <td class=xl68></td>
 
  </tr>
  </tr>
  <tr>
  <tr>
Line 120: Line 92:
   <td class=xl70 style='border-top:none;border-left:none'>CCAGACCGTTCAGCTAGATATTACG</td>
   <td class=xl70 style='border-top:none;border-left:none'>CCAGACCGTTCAGCTAGATATTACG</td>
   <td class=xl76 style='border-top:none;border-left:none'>&nbsp;</td>
   <td class=xl76 style='border-top:none;border-left:none'>&nbsp;</td>
-
  <td class=xl68></td>
 
-
  <td class=xl68></td>
 
  </tr>
  </tr>
  <tr>
  <tr>
Line 128: Line 98:
   <td class=xl70 style='border-top:none;border-left:none'>CGTAATATCTAGCTGAACGGTCTGG</td>
   <td class=xl70 style='border-top:none;border-left:none'>CGTAATATCTAGCTGAACGGTCTGG</td>
   <td class=xl76 style='border-top:none;border-left:none'>&nbsp;</td>
   <td class=xl76 style='border-top:none;border-left:none'>&nbsp;</td>
-
  <td class=xl68></td>
 
-
  <td class=xl68></td>
 
  </tr>
  </tr>
  <tr>
  <tr>
Line 136: Line 104:
   <td class=xl70 style='border-top:none;border-left:none'>GCTCATCCGGAGTTCCGTATGGC</td>
   <td class=xl70 style='border-top:none;border-left:none'>GCTCATCCGGAGTTCCGTATGGC</td>
   <td class=xl76 style='border-top:none;border-left:none'>&nbsp;</td>
   <td class=xl76 style='border-top:none;border-left:none'>&nbsp;</td>
-
  <td class=xl68></td>
 
-
  <td class=xl68></td>
 
  </tr>
  </tr>
  <tr>
  <tr>
Line 144: Line 110:
   <td class=xl70 style='border-top:none;border-left:none'>GCCATACGGAACTCCGGATGAGC</td>
   <td class=xl70 style='border-top:none;border-left:none'>GCCATACGGAACTCCGGATGAGC</td>
   <td class=xl76 style='border-top:none;border-left:none'>&nbsp;</td>
   <td class=xl76 style='border-top:none;border-left:none'>&nbsp;</td>
-
  <td class=xl68></td>
 
-
  <td class=xl68></td>
 
  </tr>
  </tr>
  <tr>
  <tr>
Line 152: Line 116:
   <td class=xl70 style='border-top:none;border-left:none'>CGACGCTCGCTCCAGCTAGGGCTTCCTTAC</td>
   <td class=xl70 style='border-top:none;border-left:none'>CGACGCTCGCTCCAGCTAGGGCTTCCTTAC</td>
   <td class=xl76 style='border-top:none;border-left:none'>&nbsp;</td>
   <td class=xl76 style='border-top:none;border-left:none'>&nbsp;</td>
-
  <td class=xl68></td>
 
-
  <td class=xl68></td>
 
  </tr>
  </tr>
  <tr>
  <tr>
Line 160: Line 122:
   <td class=xl70 style='border-top:none;border-left:none'>GTAAGGAAGCCCTAGCTGGAGCGAGCGTCG</td>
   <td class=xl70 style='border-top:none;border-left:none'>GTAAGGAAGCCCTAGCTGGAGCGAGCGTCG</td>
   <td class=xl76 style='border-top:none;border-left:none'>&nbsp;</td>
   <td class=xl76 style='border-top:none;border-left:none'>&nbsp;</td>
-
  <td class=xl68></td>
 
-
  <td class=xl68></td>
 
  </tr>
  </tr>
  <tr>
  <tr>
Line 168: Line 128:
   <td class=xl70 style='border-top:none;border-left:none'>TTCCGGCACCTACAGCGAGGAGTTTTG</td>
   <td class=xl70 style='border-top:none;border-left:none'>TTCCGGCACCTACAGCGAGGAGTTTTG</td>
   <td class=xl76 style='border-top:none;border-left:none'>&nbsp;</td>
   <td class=xl76 style='border-top:none;border-left:none'>&nbsp;</td>
-
  <td class=xl68></td>
 
-
  <td class=xl68></td>
 
  </tr>
  </tr>
  <tr>
  <tr>
Line 176: Line 134:
   <td class=xl70 style='border-top:none;border-left:none'>CAAAACTCCTCGCTGTAGGTGCCGGAA</td>
   <td class=xl70 style='border-top:none;border-left:none'>CAAAACTCCTCGCTGTAGGTGCCGGAA</td>
   <td class=xl76 style='border-top:none;border-left:none'>&nbsp;</td>
   <td class=xl76 style='border-top:none;border-left:none'>&nbsp;</td>
-
  <td class=xl68></td>
 
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  </tr>
  </tr>
  <tr>
  <tr>
Line 184: Line 140:
   <td class=xl70 style='border-top:none;border-left:none'>GGATGAGTTTTCTAGAGGCCGGTCTCC</td>
   <td class=xl70 style='border-top:none;border-left:none'>GGATGAGTTTTCTAGAGGCCGGTCTCC</td>
   <td class=xl76 style='border-top:none;border-left:none'>&nbsp;</td>
   <td class=xl76 style='border-top:none;border-left:none'>&nbsp;</td>
-
  <td class=xl68></td>
 
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  </tr>
  </tr>
  <tr>
  <tr>
Line 192: Line 146:
   <td class=xl70 style='border-top:none;border-left:none'>GGAGACCGGCCTCTAGAAAACTCATCC</td>
   <td class=xl70 style='border-top:none;border-left:none'>GGAGACCGGCCTCTAGAAAACTCATCC</td>
   <td class=xl76 style='border-top:none;border-left:none'>&nbsp;</td>
   <td class=xl76 style='border-top:none;border-left:none'>&nbsp;</td>
-
  <td class=xl68></td>
 
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  </tr>
  </tr>
  <tr>
  <tr>
Line 200: Line 152:
   <td class=xl69 style='border-top:none;border-left:none'>CGGGGCTGGCTTAACTAT</td>
   <td class=xl69 style='border-top:none;border-left:none'>CGGGGCTGGCTTAACTAT</td>
   <td class=xl76 style='border-top:none;border-left:none'>&nbsp;</td>
   <td class=xl76 style='border-top:none;border-left:none'>&nbsp;</td>
-
  <td class=xl68></td>
 
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  </tr>
  </tr>
  <tr>
  <tr>
Line 208: Line 158:
   <td class=xl69 style='border-top:none;border-left:none'>GTAAATTGAAGCTTGCATGC</td>
   <td class=xl69 style='border-top:none;border-left:none'>GTAAATTGAAGCTTGCATGC</td>
   <td class=xl76 style='border-top:none;border-left:none'>&nbsp;</td>
   <td class=xl76 style='border-top:none;border-left:none'>&nbsp;</td>
-
  <td class=xl68></td>
 
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  <td class=xl68></td>
 
  </tr>
  </tr>
  <tr>
  <tr>
   <td height=21 class=xl75 style='height:15.75pt;border-top:none'>iMR25</td>
   <td height=21 class=xl75 style='height:15.75pt;border-top:none'>iMR25</td>
-
   <td class=xl65 style='border-top:none;border-left:none'>Seq_T_NR und
+
   <td class=xl65 style='border-top:none;border-left:none'>Seq_T_NR,
   P_fcpB_For</td>
   P_fcpB_For</td>
   <td class=xl69 style='border-top:none;border-left:none'>ACTTGTGCGAACGGAATT</td>
   <td class=xl69 style='border-top:none;border-left:none'>ACTTGTGCGAACGGAATT</td>
   <td class=xl76 style='border-top:none;border-left:none'>&nbsp;</td>
   <td class=xl76 style='border-top:none;border-left:none'>&nbsp;</td>
-
  <td class=xl68></td>
 
-
  <td class=xl68></td>
 
  </tr>
  </tr>
  <tr>
  <tr>
   <td height=21 class=xl75 style='height:15.75pt;border-top:none'>iMR26</td>
   <td height=21 class=xl75 style='height:15.75pt;border-top:none'>iMR26</td>
-
   <td class=xl65 style='border-top:none;border-left:none'>Seq_T_NR und
+
   <td class=xl65 style='border-top:none;border-left:none'>Seq_T_NR,
   P_fcpB_Rev</td>
   P_fcpB_Rev</td>
   <td class=xl69 style='border-top:none;border-left:none'>CACCGGCGAAGTCGTCCT</td>
   <td class=xl69 style='border-top:none;border-left:none'>CACCGGCGAAGTCGTCCT</td>
   <td class=xl76 style='border-top:none;border-left:none'>&nbsp;</td>
   <td class=xl76 style='border-top:none;border-left:none'>&nbsp;</td>
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  <td class=xl68></td>
 
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  <tr>
Line 234: Line 178:
   <td class=xl69 style='border-top:none;border-left:none'>CTACGACGCTCGCTCCAG</td>
   <td class=xl69 style='border-top:none;border-left:none'>CTACGACGCTCGCTCCAG</td>
   <td class=xl76 style='border-top:none;border-left:none'>&nbsp;</td>
   <td class=xl76 style='border-top:none;border-left:none'>&nbsp;</td>
-
  <td class=xl68></td>
 
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  </tr>
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Line 242: Line 184:
   <td class=xl69 style='border-top:none;border-left:none'>GGCATCCATGGTGAAGACGA</td>
   <td class=xl69 style='border-top:none;border-left:none'>GGCATCCATGGTGAAGACGA</td>
   <td class=xl76 style='border-top:none;border-left:none'>&nbsp;</td>
   <td class=xl76 style='border-top:none;border-left:none'>&nbsp;</td>
-
  <td class=xl68></td>
 
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  </tr>
  </tr>
  <tr>
  <tr>
Line 250: Line 190:
   <td class=xl69 style='border-top:none;border-left:none'>AGATCGGCGAGCAGCCGT</td>
   <td class=xl69 style='border-top:none;border-left:none'>AGATCGGCGAGCAGCCGT</td>
   <td class=xl76 style='border-top:none;border-left:none'>&nbsp;</td>
   <td class=xl76 style='border-top:none;border-left:none'>&nbsp;</td>
-
  <td class=xl68></td>
 
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  <td class=xl68></td>
 
  </tr>
  </tr>
  <tr>
  <tr>
Line 258: Line 196:
   <td class=xl69 style='border-top:none;border-left:none'>CCCGCGCGTTGGCCGATT</td>
   <td class=xl69 style='border-top:none;border-left:none'>CCCGCGCGTTGGCCGATT</td>
   <td class=xl76 style='border-top:none;border-left:none'>&nbsp;</td>
   <td class=xl76 style='border-top:none;border-left:none'>&nbsp;</td>
-
  <td class=xl68></td>
 
-
  <td class=xl68></td>
 
  </tr>
  </tr>
  <tr height=22 style='height:16.5pt'>
  <tr height=22 style='height:16.5pt'>
Line 266: Line 202:
   <td class=xl69 style='border-top:none;border-left:none'>GTTTCTTCGAATTCGCGGCCGCTTCTAGAATGGAAACCCCAGCGCAGCTT</td>
   <td class=xl69 style='border-top:none;border-left:none'>GTTTCTTCGAATTCGCGGCCGCTTCTAGAATGGAAACCCCAGCGCAGCTT</td>
   <td class=xl77 style='border-top:none;border-left:none'>&nbsp;</td>
   <td class=xl77 style='border-top:none;border-left:none'>&nbsp;</td>
-
  <td class=xl68></td>
 
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  </tr>
  <tr height=22 style='height:16.5pt'>
  <tr height=22 style='height:16.5pt'>
Line 274: Line 208:
   <td class=xl69 style='border-top:none;border-left:none'>GTTTCTTCCTGCAGCGGCCGCTACTAGTCTAACACTCTCCACGGTTGAAGCTC</td>
   <td class=xl69 style='border-top:none;border-left:none'>GTTTCTTCCTGCAGCGGCCGCTACTAGTCTAACACTCTCCACGGTTGAAGCTC</td>
   <td class=xl77 style='border-top:none;border-left:none'>&nbsp;</td>
   <td class=xl77 style='border-top:none;border-left:none'>&nbsp;</td>
-
  <td class=xl68></td>
 
-
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  </tr>
  </tr>
  <tr height=22 style='height:16.5pt'>
  <tr height=22 style='height:16.5pt'>
Line 282: Line 214:
   <td class=xl69 style='border-top:none;border-left:none'>GTTTCTTCGAATTCGCGGCCGCTTCTAGAATGGAGTTTGGGCTCAGCTGG</td>
   <td class=xl69 style='border-top:none;border-left:none'>GTTTCTTCGAATTCGCGGCCGCTTCTAGAATGGAGTTTGGGCTCAGCTGG</td>
   <td class=xl77 style='border-top:none;border-left:none'>&nbsp;</td>
   <td class=xl77 style='border-top:none;border-left:none'>&nbsp;</td>
-
  <td class=xl68></td>
 
-
  <td class=xl68></td>
 
  </tr>
  </tr>
  <tr height=22 style='height:16.5pt'>
  <tr height=22 style='height:16.5pt'>
Line 290: Line 220:
   <td class=xl69 style='border-top:none;border-left:none'>GTTTCTTCCTGCAGCGGCCGCTACTAGTTCATTTACCCGGAGACAGGGAG</td>
   <td class=xl69 style='border-top:none;border-left:none'>GTTTCTTCCTGCAGCGGCCGCTACTAGTTCATTTACCCGGAGACAGGGAG</td>
   <td class=xl77 style='border-top:none;border-left:none'>&nbsp;</td>
   <td class=xl77 style='border-top:none;border-left:none'>&nbsp;</td>
-
  <td class=xl68></td>
 
-
  <td class=xl68></td>
 
  </tr>
  </tr>
  <tr>
  <tr>
   <td height=21 class=xl75 style='height:15.75pt;border-top:none'>iMR35</td>
   <td height=21 class=xl75 style='height:15.75pt;border-top:none'>iMR35</td>
   <td class=xl65 style='border-top:none;border-left:none'>Seq_pJET12_for</td>
   <td class=xl65 style='border-top:none;border-left:none'>Seq_pJET12_for</td>
-
   <td class=xl69 style='border-top:none;border-left:none'>cggcgtaatacgactcactata</td>
+
   <td class=xl69 style='border-top:none;border-left:none'>CGGCGTAATACGACTCACTATA</td>
   <td class=xl76 style='border-top:none;border-left:none'>&nbsp;</td>
   <td class=xl76 style='border-top:none;border-left:none'>&nbsp;</td>
-
  <td class=xl68></td>
 
-
  <td class=xl68></td>
 
  </tr>
  </tr>
  <tr>
  <tr>
   <td height=21 class=xl75 style='height:15.75pt;border-top:none'>iMR36</td>
   <td height=21 class=xl75 style='height:15.75pt;border-top:none'>iMR36</td>
   <td class=xl65 style='border-top:none;border-left:none'>Seq_pJET12_rev</td>
   <td class=xl65 style='border-top:none;border-left:none'>Seq_pJET12_rev</td>
-
   <td class=xl69 style='border-top:none;border-left:none'>acctacaacggttcctgatg</td>
+
   <td class=xl69 style='border-top:none;border-left:none'>ACCTACAACGGTTCCTGATG</td>
   <td class=xl76 style='border-top:none;border-left:none'>&nbsp;</td>
   <td class=xl76 style='border-top:none;border-left:none'>&nbsp;</td>
-
  <td class=xl68></td>
 
-
  <td class=xl68></td>
 
  </tr>
  </tr>
  <tr>
  <tr>
   <td height=21 class=xl75 style='height:15.75pt;border-top:none'>iMR37</td>
   <td height=21 class=xl75 style='height:15.75pt;border-top:none'>iMR37</td>
-
   <td class=xl65 style='border-top:none;border-left:none'>sequencing_forward_primer_(VF2)</td>
+
   <td class=xl65 style='border-top:none;border-left:none'>Seq_for (VF2)</td>
-
   <td class=xl70 style='border-top:none;border-left:none'>tgccacctgacgtctaagaa</td>
+
   <td class=xl70 style='border-top:none;border-left:none'>TGCCACCTGACGTCTAAGAA</td>
   <td class=xl76 style='border-top:none;border-left:none'>&nbsp;</td>
   <td class=xl76 style='border-top:none;border-left:none'>&nbsp;</td>
-
  <td class=xl68></td>
 
-
  <td class=xl68></td>
 
  </tr>
  </tr>
  <tr>
  <tr>
   <td height=21 class=xl75 style='height:15.75pt;border-top:none'>iMR38</td>
   <td height=21 class=xl75 style='height:15.75pt;border-top:none'>iMR38</td>
-
   <td class=xl65 style='border-top:none;border-left:none'>sequencing_reverse_primer_(VR)</td>
+
   <td class=xl65 style='border-top:none;border-left:none'>Seq_rev (VR)</td>
-
   <td class=xl70 style='border-top:none;border-left:none'>attaccgcctttgagtgagc</td>
+
   <td class=xl70 style='border-top:none;border-left:none'>ATTACCGCCTTTGAGTGAGC</td>
   <td class=xl76 style='border-top:none;border-left:none'>&nbsp;</td>
   <td class=xl76 style='border-top:none;border-left:none'>&nbsp;</td>
-
  <td class=xl68></td>
 
-
  <td class=xl68></td>
 
  </tr>
  </tr>
  <tr>
  <tr>
Line 330: Line 250:
   <td class=xl69 style='border-top:none;border-left:none'>GCTCATGGAAAACGGTGTAA</td>
   <td class=xl69 style='border-top:none;border-left:none'>GCTCATGGAAAACGGTGTAA</td>
   <td class=xl76 style='border-top:none;border-left:none'>&nbsp;</td>
   <td class=xl76 style='border-top:none;border-left:none'>&nbsp;</td>
-
  <td class=xl68></td>
 
-
  <td class=xl68></td>
 
  </tr>
  </tr>
  <tr>
  <tr>
Line 338: Line 256:
   <td class=xl69 style='border-top:none;border-left:none'>GTTTCTTCGAATTCGCGGCCGCTTCTAGAATGGTGAGCAAGGGCGAGG</td>
   <td class=xl69 style='border-top:none;border-left:none'>GTTTCTTCGAATTCGCGGCCGCTTCTAGAATGGTGAGCAAGGGCGAGG</td>
   <td class=xl76 style='border-top:none;border-left:none'>&nbsp;</td>
   <td class=xl76 style='border-top:none;border-left:none'>&nbsp;</td>
-
  <td class=xl68></td>
 
-
  <td class=xl68></td>
 
  </tr>
  </tr>
  <tr>
  <tr>
   <td height=21 class=xl75 style='height:15.75pt;border-top:none'>iMR41</td>
   <td height=21 class=xl75 style='height:15.75pt;border-top:none'>iMR41</td>
   <td class=xl65 style='border-top:none;border-left:none'>eGFP_rev</td>
   <td class=xl65 style='border-top:none;border-left:none'>eGFP_rev</td>
-
   <td class=xl69 style='border-top:none;border-left:none'><font class="font9">GTTTCTTCCTGCAGCGGCCGCTACTAGT</font><font
+
   <td class=xl69 style='border-top:none;border-left:none'>GTTTCTTCCTGCAGCGGCCGCTACTAGTTTACTTGTACAGCTCGTCC</td>
-
  class="font7">TTACTTGTACAGCTCGTCC</font></td>
+
   <td class=xl78 style='border-top:none;border-left:none'>&nbsp;</td>
   <td class=xl78 style='border-top:none;border-left:none'>&nbsp;</td>
-
  <td class=xl68></td>
 
-
  <td class=xl68></td>
 
  </tr>
  </tr>
  <tr>
  <tr>
   <td height=21 class=xl75 style='height:15.75pt;border-top:none'>iMR42</td>
   <td height=21 class=xl75 style='height:15.75pt;border-top:none'>iMR42</td>
   <td class=xl65 style='border-top:none;border-left:none'>SPTP1_for</td>
   <td class=xl65 style='border-top:none;border-left:none'>SPTP1_for</td>
-
   <td class=xl71 style='border-top:none;border-left:none'><font class="font11">GTTTCTTCGAATTCGCGGCCGCTTCTAGA</font><font
+
   <td class=xl71 style='border-top:none;border-left:none'>GTTTCTTCGAATTCGCGGCCGCTTCTAGAATGAGATCCTTTTGCATCG</td>
-
  class="font10">ATGAGATCCTTTTGCATCG</font></td>
+
   <td class=xl78 style='border-top:none;border-left:none'>Signal/Transitpeptid Stroma 1 AtpC</td>
-
   <td class=xl78 style='border-top:none;border-left:none'>Signal/Transitpeptid
+
-
  Stroma 1 AtpC</td>
+
-
  <td class=xl68></td>
+
-
  <td></td>
+
  </tr>
  </tr>
  <tr>
  <tr>
   <td height=21 class=xl75 style='height:15.75pt;border-top:none'>iMR43</td>
   <td height=21 class=xl75 style='height:15.75pt;border-top:none'>iMR43</td>
   <td class=xl65 style='border-top:none;border-left:none'>SPTP1_rev</td>
   <td class=xl65 style='border-top:none;border-left:none'>SPTP1_rev</td>
-
   <td class=xl71 style='border-top:none;border-left:none'><font class="font11">GTTTCTTCCTGCAGCGGCCGCTACTAGT</font><font
+
   <td class=xl71 style='border-top:none;border-left:none'>GTTTCTTCCTGCAGCGGCCGCTACTAGTTCCATCCATGACAATCGTTG</td>
-
  class="font10">TCCATCCATGACAATCGTTG</font></td>
+
   <td class=xl78 style='border-top:none;border-left:none'>Signal/Transitpeptid Stroma 1 AtpC</td>
-
   <td class=xl78 style='border-top:none;border-left:none'>Signal/Transitpeptid
+
-
  Stroma 1 AtpC</td>
+
-
  <td></td>
+
-
  <td></td>
+
  </tr>
  </tr>
  <tr>
  <tr>
   <td height=21 class=xl75 style='height:15.75pt;border-top:none'>iMR44</td>
   <td height=21 class=xl75 style='height:15.75pt;border-top:none'>iMR44</td>
   <td class=xl65 style='border-top:none;border-left:none'>SPTP2_for</td>
   <td class=xl65 style='border-top:none;border-left:none'>SPTP2_for</td>
-
   <td class=xl71 style='border-top:none;border-left:none'><font class="font11">GTTTCTTCGAATTCGCGGCCGCTTCTAGA</font><font
+
   <td class=xl71 style='border-top:none;border-left:none'>GTTTCTTCGAATTCGCGGCCGCTTCTAGAATGAAGACTGCTGTCATTGC</td>
-
  class="font10">ATGAAGACTGCTGTCATTGC</font></td>
+
   <td class=xl78 style='border-top:none;border-left:none'>Signal/Transitpeptid Stroma 2 FCpD</td>
-
   <td class=xl78 style='border-top:none;border-left:none'>Signal/Transitpeptid
+
-
  Stroma 2 FCpD</td>
+
-
  <td></td>
+
-
  <td></td>
+
  </tr>
  </tr>
  <tr>
  <tr>
   <td height=21 class=xl75 style='height:15.75pt;border-top:none'>iMR45</td>
   <td height=21 class=xl75 style='height:15.75pt;border-top:none'>iMR45</td>
   <td class=xl65 style='border-top:none;border-left:none'>SPTP2_rev</td>
   <td class=xl65 style='border-top:none;border-left:none'>SPTP2_rev</td>
-
   <td class=xl71 style='border-top:none;border-left:none'><font class="font11">GTTTCTTCCTGCAGCGGCCGCTACTAGT</font><font
+
   <td class=xl71 style='border-top:none;border-left:none'>GTTTCTTCCTGCAGCGGCCGCTACTAGTGGTGGCAACCGAAGTACGGG</td>
-
  class="font10">GGTGGCAACCGAAGTACGGG</font></td>
+
   <td class=xl78 style='border-top:none;border-left:none'>Signal/Transitpeptid Stroma 2 FCpD</td>
-
   <td class=xl78 style='border-top:none;border-left:none'>Signal/Transitpeptid
+
-
  Stroma 2 FCpD</td>
+
-
  <td></td>
+
-
  <td></td>
+
  </tr>
  </tr>
  <tr>
  <tr>
   <td height=21 class=xl75 style='height:15.75pt;border-top:none'>iMR46</td>
   <td height=21 class=xl75 style='height:15.75pt;border-top:none'>iMR46</td>
   <td class=xl65 style='border-top:none;border-left:none'>SP1_for</td>
   <td class=xl65 style='border-top:none;border-left:none'>SP1_for</td>
-
   <td class=xl71 style='border-top:none;border-left:none'><font class="font11">GTTTCTTCGAATTCGCGGCCGCTTCTAGA</font><font
+
   <td class=xl71 style='border-top:none;border-left:none'>GTTTCTTCGAATTCGCGGCCGCTTCTAGAATGACTGCAATAGGGTCTTT</td>
-
  class="font10">ATGACTGCAATAGGGTCTTT</font></td>
+
   <td class=xl78 style='border-top:none;border-left:none'>Signalpeptid ER 1 PDI</td>
   <td class=xl78 style='border-top:none;border-left:none'>Signalpeptid ER 1 PDI</td>
-
  <td></td>
 
-
  <td></td>
 
  </tr>
  </tr>
  <tr>
  <tr>
   <td height=21 class=xl75 style='height:15.75pt;border-top:none'>iMR47</td>
   <td height=21 class=xl75 style='height:15.75pt;border-top:none'>iMR47</td>
   <td class=xl65 style='border-top:none;border-left:none'>SP1_rev</td>
   <td class=xl65 style='border-top:none;border-left:none'>SP1_rev</td>
-
   <td class=xl71 style='border-top:none;border-left:none'><font class="font11">GTTTCTTCCTGCAGCGGCCGCTACTAGT</font><font
+
   <td class=xl71 style='border-top:none;border-left:none'>GTTTCTTCCTGCAGCGGCCGCTACTAGTGGCAAGACACACTGCTGTG</td>
-
  class="font10">GGCAAGACACACTGCTGTG</font></td>
+
   <td class=xl78 style='border-top:none;border-left:none'>Signalpeptid ER 1 PDI</td>
   <td class=xl78 style='border-top:none;border-left:none'>Signalpeptid ER 1 PDI</td>
-
  <td></td>
 
-
  <td></td>
 
  </tr>
  </tr>
  <tr>
  <tr>
   <td height=21 class=xl75 style='height:15.75pt;border-top:none'>iMR48</td>
   <td height=21 class=xl75 style='height:15.75pt;border-top:none'>iMR48</td>
   <td class=xl65 style='border-top:none;border-left:none'>SP2_for</td>
   <td class=xl65 style='border-top:none;border-left:none'>SP2_for</td>
-
   <td class=xl71 style='border-top:none;border-left:none'><font class="font11">GTTTCTTCGAATTCGCGGCCGCTTCTAGA</font><font
+
   <td class=xl71 style='border-top:none;border-left:none'>GTTTCTTCGAATTCGCGGCCGCTTCTAGAATGCGCTCGTTTCTACTTTG</td>
-
  class="font10">ATGCGCTCGTTTCTACTTTG</font></td>
+
   <td class=xl78 style='border-top:none;border-left:none'>Signalpeptid ER 2 CA-VI</td>
-
   <td class=xl78 style='border-top:none;border-left:none'>Signalpeptid ER 2
+
-
  CA-VI</td>
+
-
  <td></td>
+
-
  <td></td>
+
  </tr>
  </tr>
  <tr>
  <tr>
   <td height=21 class=xl75 style='height:15.75pt;border-top:none'>iMR49</td>
   <td height=21 class=xl75 style='height:15.75pt;border-top:none'>iMR49</td>
   <td class=xl65 style='border-top:none;border-left:none'>SP2_rev</td>
   <td class=xl65 style='border-top:none;border-left:none'>SP2_rev</td>
-
   <td class=xl71 style='border-top:none;border-left:none'><font class="font11">GTTTCTTCCTGCAGCGGCCGCTACTAGT</font><font
+
   <td class=xl71 style='border-top:none;border-left:none'>GTTTCTTCCTGCAGCGGCCGCTACTAGTAGCAGTCGCGAACGAAGCG</td>
-
  class="font10">AGCAGTCGCGAACGAAGCG</font></td>
+
   <td class=xl78 style='border-top:none;border-left:none'>Signalpeptid ER 2 CA-VI</td>
-
   <td class=xl78 style='border-top:none;border-left:none'>Signalpeptid ER 2
+
-
  CA-VI</td>
+
-
  <td></td>
+
-
  <td></td>
+
  </tr>
  </tr>
  <tr height=22 style='height:16.5pt'>
  <tr height=22 style='height:16.5pt'>
   <td height=22 class=xl75 style='height:16.5pt;border-top:none'>iMR50</td>
   <td height=22 class=xl75 style='height:16.5pt;border-top:none'>iMR50</td>
   <td class=xl67 style='border-top:none;border-left:none'>RFP_for</td>
   <td class=xl67 style='border-top:none;border-left:none'>RFP_for</td>
-
   <td class=xl71 style='border-top:none;border-left:none'><font class="font12">GTTTCTTCGAATTCGCGGCCGCTTCTAGA</font><font
+
   <td class=xl71 style='border-top:none;border-left:none'>GTTTCTTCGAATTCGCGGCCGCTTCTAGAATGGCTTCCTCCGAAGACG</td>
-
  class="font10">atggcttcctccgaagacg</font></td>
+
   <td class=xl78 style='border-top:none;border-left:none'>&nbsp;</td>
   <td class=xl78 style='border-top:none;border-left:none'>&nbsp;</td>
-
  <td></td>
 
-
  <td></td>
 
  </tr>
  </tr>
  <tr>
  <tr>
   <td height=21 class=xl75 style='height:15.75pt;border-top:none'>iMR51</td>
   <td height=21 class=xl75 style='height:15.75pt;border-top:none'>iMR51</td>
   <td class=xl67 style='border-top:none;border-left:none'>RFP_rev</td>
   <td class=xl67 style='border-top:none;border-left:none'>RFP_rev</td>
-
   <td class=xl71 style='border-top:none;border-left:none'>ATATGGATCCagcaccggtggagtgacg</td>
+
   <td class=xl71 style='border-top:none;border-left:none'>ATATGGATCCAGCACCGGTGGAGTGACG</td>
   <td class=xl78 style='border-top:none;border-left:none'>&nbsp;</td>
   <td class=xl78 style='border-top:none;border-left:none'>&nbsp;</td>
   <td></td>
   <td></td>
Line 465: Line 345:
   <td height=22 class=xl79 style='height:16.5pt;border-top:none'>iMR54</td>
   <td height=22 class=xl79 style='height:16.5pt;border-top:none'>iMR54</td>
   <td class=xl80 style='border-top:none;border-left:none'>RBS_for</td>
   <td class=xl80 style='border-top:none;border-left:none'>RBS_for</td>
-
   <td class=xl81 style='border-top:none;border-left:none'>aaagaggagaaa</td>
+
   <td class=xl81 style='border-top:none;border-left:none'>AAAGAGGAGAAA</td>
   <td class=xl82 style='border-top:none;border-left:none'>Colonie-PCR PlacI-RBS</td>
   <td class=xl82 style='border-top:none;border-left:none'>Colonie-PCR PlacI-RBS</td>
   <td></td>
   <td></td>

Revision as of 13:22, 4 October 2013

Primer list

No Name Sequence Comment
iMR01 ble-for GTTTCTTCGAATTCGCGGCCGCTTCTAGAATGGCCAAGTTGACCAGTGCCGTTC  
iMR02 ble-rev GTTTCTTCCTGCAGCGGCCGCTACTAGTTCAGTCCTGCTCCTCGGCCACGAAG  
iMR03 cat-for GTTTCTTCGAATTCGCGGCCGCTTCTAGAATGGAGAAAAAAATCACTGGATATA  
iMR04 cat-rev GTTTCTTCCTGCAGCGGCCGCTACTAGTTTACGCCCCGCCCTGCCACTCATCGC  
iMR05 P_fcbP-for GTTTCTTCGAATTCGCGGCCGCTTCTAGAATCCGGAGAGATTCAAAATTCAATC  
iMR06 P_fcbP-rev GTTTCTTCCTGCAGCGGCCGCTACTAGTCTTGACATCTGGCAACCGTGAAATA  
iMR07 P_NR-for GTTTCTTCGAATTCGCGGCCGCTTCTAGACATATGCGGAAGTGACTGTAAACGA  
iMR08 P_NR-rev GTTTCTTCCTGCAGCGGCCGCTACTAGTCGTTCGCACAAGTGGTGACTTTGAA  
iMR09 T_fcpA-for GTTTCTTCGAATTCGCGGCCGCTTCTAGACCTTCCTTAAAAATTTAATTTTCAT  
iMR10 T_fcpA-rev GTTTCTTCCTGCAGCGGCCGCTACTAGTCTCGAGAAAAC TCATCCTGTG CCTT  
iMR11 T_NR-for GTTTCTTCGAATTCGCGGCCGCTTCTAGACAATTTACTTTAAGACTAGC  
iMR12 T_NR-rev GTTTCTTCCTGCAGCGGCCGCTACTAGTTCCGGATGCGTTCATTTTAG  
iMR13 Cat-bb-Pvu-For CCAGACCGTTCAGCTAGATATTACG  
iMR14 Cat-bb-Pvu-Rev CGTAATATCTAGCTGAACGGTCTGG  
iMR15 Cat-bb-Eco-For GCTCATCCGGAGTTCCGTATGGC  
iMR16 Cat-bb-Eco-Rev GCCATACGGAACTCCGGATGAGC  
iMR17 PfcpB-Pvu-For CGACGCTCGCTCCAGCTAGGGCTTCCTTAC  
iMR18 PfcpB-Pvu-Rev GTAAGGAAGCCCTAGCTGGAGCGAGCGTCG  
iMR19 PNR-Pst-For TTCCGGCACCTACAGCGAGGAGTTTTG  
iMR20 PNR-Pst-Rev CAAAACTCCTCGCTGTAGGTGCCGGAA  
iMR21 T_fcpa-Xho-For GGATGAGTTTTCTAGAGGCCGGTCTCC  
iMR22 T-fcpa-Xho-Rev GGAGACCGGCCTCTAGAAAACTCATCC  
iMR23 Seq_P_NR_For CGGGGCTGGCTTAACTAT  
iMR24 Seq_P_NR_Rev GTAAATTGAAGCTTGCATGC  
iMR25 Seq_T_NR, P_fcpB_For ACTTGTGCGAACGGAATT  
iMR26 Seq_T_NR, P_fcpB_Rev CACCGGCGAAGTCGTCCT  
iMR27 Seq_Ble_For CTACGACGCTCGCTCCAG  
iMR28 Seq_Ble_Rev GGCATCCATGGTGAAGACGA  
iMR29 Seq_T_fcpA_For AGATCGGCGAGCAGCCGT  
iMR30 Seq_T_fcpA_Rev CCCGCGCGTTGGCCGATT  
iMR31 Cl4mA_l_for GTTTCTTCGAATTCGCGGCCGCTTCTAGAATGGAAACCCCAGCGCAGCTT  
iMR32 Cl4mA_l_rev GTTTCTTCCTGCAGCGGCCGCTACTAGTCTAACACTCTCCACGGTTGAAGCTC  
iMR33 Cl4mA_h_for GTTTCTTCGAATTCGCGGCCGCTTCTAGAATGGAGTTTGGGCTCAGCTGG  
iMR34 Cl4mA_h_rev GTTTCTTCCTGCAGCGGCCGCTACTAGTTCATTTACCCGGAGACAGGGAG  
iMR35 Seq_pJET12_for CGGCGTAATACGACTCACTATA  
iMR36 Seq_pJET12_rev ACCTACAACGGTTCCTGATG  
iMR37 Seq_for (VF2) TGCCACCTGACGTCTAAGAA  
iMR38 Seq_rev (VR) ATTACCGCCTTTGAGTGAGC  
iMR39 Seq_cat.int_rev GCTCATGGAAAACGGTGTAA  
iMR40 eGFP_for GTTTCTTCGAATTCGCGGCCGCTTCTAGAATGGTGAGCAAGGGCGAGG  
iMR41 eGFP_rev GTTTCTTCCTGCAGCGGCCGCTACTAGTTTACTTGTACAGCTCGTCC  
iMR42 SPTP1_for GTTTCTTCGAATTCGCGGCCGCTTCTAGAATGAGATCCTTTTGCATCG Signal/Transitpeptid Stroma 1 AtpC
iMR43 SPTP1_rev GTTTCTTCCTGCAGCGGCCGCTACTAGTTCCATCCATGACAATCGTTG Signal/Transitpeptid Stroma 1 AtpC
iMR44 SPTP2_for GTTTCTTCGAATTCGCGGCCGCTTCTAGAATGAAGACTGCTGTCATTGC Signal/Transitpeptid Stroma 2 FCpD
iMR45 SPTP2_rev GTTTCTTCCTGCAGCGGCCGCTACTAGTGGTGGCAACCGAAGTACGGG Signal/Transitpeptid Stroma 2 FCpD
iMR46 SP1_for GTTTCTTCGAATTCGCGGCCGCTTCTAGAATGACTGCAATAGGGTCTTT Signalpeptid ER 1 PDI
iMR47 SP1_rev GTTTCTTCCTGCAGCGGCCGCTACTAGTGGCAAGACACACTGCTGTG Signalpeptid ER 1 PDI
iMR48 SP2_for GTTTCTTCGAATTCGCGGCCGCTTCTAGAATGCGCTCGTTTCTACTTTG Signalpeptid ER 2 CA-VI
iMR49 SP2_rev GTTTCTTCCTGCAGCGGCCGCTACTAGTAGCAGTCGCGAACGAAGCG Signalpeptid ER 2 CA-VI
iMR50 RFP_for GTTTCTTCGAATTCGCGGCCGCTTCTAGAATGGCTTCCTCCGAAGACG  
iMR51 RFP_rev ATATGGATCCAGCACCGGTGGAGTGACG  
iMR52 MTS_for ATATGGATCCGCGCCGCAGCGGGAGGAAG  
iMR53 MTS_rev GTTTCTTCCTGCAGCGGCCGCTACTAGTCTACCTTTCTAGGAAAAGTTGGCGC  
iMR54 RBS_for AAAGAGGAGAAA Colonie-PCR PlacI-RBS

Retrieved from "http://2013.igem.org/Team:Marburg/Notebook:Primer_list"