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Team:Paris Saclay/Notebook/August/6
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(Difference between revisions)
(→1 - Electrophoresis to check the PCR Colonies products : BBa_K1155004, BBa_K1155005, BBa_K1155006) |
(→2 - Liquid culture of DH5α with BBa_K1155004, BBa_K1155005 and BBa_K1155006) |
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| - | ====2 - Liquid culture of | + | ====2 - Liquid culture of DH5αstrain containing BBa_K1155004, BBa_K1155005 and BBa_K1155006 ==== |
Xiaoing, Anaïs | Xiaoing, Anaïs | ||
| Line 78: | Line 78: | ||
Used quantities : | Used quantities : | ||
* LB : 5mL | * LB : 5mL | ||
| - | * | + | * Chloramphenicol (1000X, 20µg/mL) : 5µL |
| - | * BBa_K1155004, BBa_K1155005 and BBa_K1155006 : 25µL | + | * Strain containing BBa_K1155004, BBa_K1155005 and BBa_K1155006 : 25µL |
| - | We | + | We incubate the cultures over night at 37°C with agigation at 180 RPM. |
| - | We | + | We picked colonies number 6, 7 and 8 for each promoter. |
===='''Objective : obtaining BBa_K1155007'''==== | ===='''Objective : obtaining BBa_K1155007'''==== | ||
Revision as of 18:34, 3 October 2013
Notebook : August 6
Lab work
A - Aerobic/Anaerobic regulation system
Objective : obtaining BBa_K1155004, BBa_K1155005, BBa_K1155006
1 - Electrophoresis to check the PCR Colonies products : BBa_K1155004, BBa_K1155005, BBa_K1155006
XiaoJing, Damir, Anaïs
- BBa_K1155004 :
 
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- BBa_K1155005 :
 
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- BBa_K1155006 :
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Expected size :
- NarK, NarG, NirB : 500 bp
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We obtain fragments at the good size for all the colonies. We will make a culture for DH5α strain containing BBa_K1155004, BBa_K1155005 and BBa_K1155006. We will also sequence our plasmids. |
2 - Liquid culture of DH5αstrain containing BBa_K1155004, BBa_K1155005 and BBa_K1155006
Xiaoing, Anaïs
Used quantities :
- LB : 5mL
- Chloramphenicol (1000X, 20µg/mL) : 5µL
- Strain containing BBa_K1155004, BBa_K1155005 and BBa_K1155006 : 25µL
We incubate the cultures over night at 37°C with agigation at 180 RPM.
We picked colonies number 6, 7 and 8 for each promoter.
Objective : obtaining BBa_K1155007
1 - Electroelution of BBa_I732017 digested by EcoRI/SpeI
Nadia
Protocol : Electroelution
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The electroelution was good. We will ligate RBS-LacZ with Term and pSB1C3. |
Objective : obtaining biobricks in pSB3K3
1 - Extraction of BBa_J04450 from DH5α
Abdou
Protocol : Low copy plamid extraction
B - PCB sensing system
Objective : obtaining BBa_K1155002
1 - Sequence analysis for BBa_K1155002 in clones 4, 17 and 22
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The sequence is good for each clone. We obtain a new biobrick : BBa_K1155002. |
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