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Team:Paris Saclay/Notebook/August/7
From 2013.igem.org
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(→2 - Tranformation BBa_K115507 in DH5α) |
(→1 - Tranduction of Km in MG1655Z1) |
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===='''1 - Tranduction of Km in MG1655Z1 ==== | ===='''1 - Tranduction of Km in MG1655Z1 ==== | ||
| - | Nadia, | + | Nadia, XiaoJing |
Protocol : [[Team:Paris_Saclay/transduction|Transduction]] | Protocol : [[Team:Paris_Saclay/transduction|Transduction]] | ||
| - | + | Wild type strain is MG1655Z1 and our mutant strain is BW : Δfnr::Km. | |
| - | + | ||
We did the first step of the protocol : bacteriophage stock which packed Km gene. | We did the first step of the protocol : bacteriophage stock which packed Km gene. | ||
Revision as of 18:58, 3 October 2013
Contents |
Notebook : August 7
Lab work
A - Aerobic/Anaerobic regulation system
Objective : obtaining BBa_K1155007
1 - Ligation of RBS-LacZ-Term in pSB1C3
Anaïs, Nadia
Used quantities :
- RBS-AmilCP : 2µL
- Term in pSB1C3 : 2.5µL
- Buffer : 1µL
- Ligase : 1µL
- H2O : 3.5µL
2 - Tranformation BBa_K115507 in DH5αstrain
XiaoJing
Protocol : Bacterial transformation
Objective : characterize BBa_K1155000, BBa_K1155004, BBa_K1155005, BBa_K1155006
1 - Tranduction of Km in MG1655Z1
Nadia, XiaoJing
Protocol : Transduction
Wild type strain is MG1655Z1 and our mutant strain is BW : Δfnr::Km.
We did the first step of the protocol : bacteriophage stock which packed Km gene.
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