Team:Bielefeld-Germany/Project/Mediators

From 2013.igem.org

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Of great interest is the production of endogenous mediators. The overexpression of glyceroldehydrogenase in E. coli is a promising approach. Because many derivates of glyceroldehydrogenase are small, water-soluble redoxmolecules, they have the properties of a mediator. Futhermore, it will be tested, if there is a possibility of expressing the mediator phenazin. Phenazin is an endogenous mediator of Pseudomonas species.  
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The production of endogenous mediators is of great interest. The overexpression of glyceroldehydrogenase in ''E. coli'' is a promising approach for production of NADH as electron-shuttle. Next to NADH as endogenous mediator we established the Riboflavin synthesis pathway in ''Escherichia coli''. Futhermore, we have tested the possibility of expressing the mediator phenazin. Phenazin is an endogenous mediator of ''Pseudomonas'' species.  
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Revision as of 09:29, 4 October 2013



Mediators


Overview

Figure 1: Principle of electron transfer from bacteria to anode via mediators as electron shuttle.

The production of endogenous mediators is of great interest. The overexpression of glyceroldehydrogenase in E. coli is a promising approach for production of NADH as electron-shuttle. Next to NADH as endogenous mediator we established the Riboflavin synthesis pathway in Escherichia coli. Futhermore, we have tested the possibility of expressing the mediator phenazin. Phenazin is an endogenous mediator of Pseudomonas species.











References

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Contents

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