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Team:UANL Mty-Mexico/Safety
From 2013.igem.org
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<h3>Potential Risks</h3> | <h3>Potential Risks</h3> | ||
<p align="justify">Given its natural habitat of the large bowel of mammals, E. coli will not likely survive for long periods in soil, water, or air. E. coli K-12 has been shown to have poorer survival characteristics in soil and water than other E. coli . The ability of E.coli to survive under environmental conditionsis thus very limited.</p> | <p align="justify">Given its natural habitat of the large bowel of mammals, E. coli will not likely survive for long periods in soil, water, or air. E. coli K-12 has been shown to have poorer survival characteristics in soil and water than other E. coli . The ability of E.coli to survive under environmental conditionsis thus very limited.</p> | ||
| - | <p><a href="https://2013.igem.org/Team:UANL_Mty-Mexico/Safety/potential_risks"><font color="#fff">More</font></a> </p> | + | <p><a href="https://2013.igem.org/Team:UANL_Mty-Mexico/Safety/potential_risks"><font color="#fff">More</font></a></p> |
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| + | <h3>Genetic Modifications</h3> | ||
| + | <p align="justify">The project “Thermocoli” consists in a circuit of transcription factors and reporter genes, some of which are under the post-transcriptional regulation of thermoregulable RNA elements, also known as RNA thermometers. These genes are arrange in a circuit constructed in such a way that three distinguishable states should emerge, characterized by the expression and repression of two different reporter fluorescent proteins.</p> | ||
| + | <p><a href="https://2013.igem.org/Team:UANL_Mty-Mexico/Safety/genetic_modifications" class="btn btn-primary"><font color="#fff">More</font></a></p> | ||
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| + | <h3>Genetic Modifications</h3> | ||
| + | <p align="justify">The project “Thermocoli” consists in a circuit of transcription factors and reporter genes, some of which are under the post-transcriptional regulation of thermoregulable RNA elements, also known as RNA thermometers. These genes are arrange in a circuit constructed in such a way that three distinguishable states should emerge, characterized by the expression and repression of two different reporter fluorescent proteins.</p> | ||
| + | <p><a href="https://2013.igem.org/Team:UANL_Mty-Mexico/Safety/genetic_modifications" class="btn btn-primary"><font color="#fff">More</font></a></p> | ||
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| + | <div class="caption"> | ||
| + | <h3>Genetic Modifications</h3> | ||
| + | <p align="justify">The project “Thermocoli” consists in a circuit of transcription factors and reporter genes, some of which are under the post-transcriptional regulation of thermoregulable RNA elements, also known as RNA thermometers. These genes are arrange in a circuit constructed in such a way that three distinguishable states should emerge, characterized by the expression and repression of two different reporter fluorescent proteins.</p> | ||
| + | <p><a href="https://2013.igem.org/Team:UANL_Mty-Mexico/Safety/genetic_modifications" class="btn btn-primary"><font color="#fff">More</font></a></p> | ||
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Revision as of 18:07, 24 October 2013
Overview
Sometimes the laboratory can be dangerous; there are many hazards that have the potential to injure or cause ill health or disease. Protecting the health and safety of people in the laboratory is a community expectation that makes good standing.
We have to understand the risks that synthetic organisms pose to the environment, what kinds of information are needed to support rigorous assessments, or who should collect such data (Dana, et al, 2012)
.- Pay attention in the differences between the function of natural and synthetic organisms and how it affect the interactions with the environment. Research in this area could, for instance, look for changes in a synthetic organism’s production of toxic substances or other harmful metabolites.
- Analyze if the escaped microorganisms have the hability to survive and compete with natural organisms, and how this will affect habitats or biodiversity.
- Another area of risk is gene transfer, is important to determine if synthetic organisms will pass on properties such as antibiotic resistance and this represent possible threats to human health.
We aim to apply the risk management approach to safety in the laboratory and this means to complete a risk assessment of this research project.
Safety forms were approved on: September 22, 2013 by Evan Appleton.
Safety: Risk Assessment
Organism Description
Escherichia coli is an Enterobacteria described by Bergey's Manual (1957). Escherichia is a Gram-negative rod which can be motile by peritrichous flagella or nonmotile. Because of its wide use as a model organism in research in microbial genetics and physiology, and its use in industrial applications, E. coli K-12 is one of the most extensively studied microorganisms.
Genetic Modifications
The project “Thermocoli” consists in a circuit of transcription factors and reporter genes, some of which are under the post-transcriptional regulation of thermoregulable RNA elements, also known as RNA thermometers. These genes are arrange in a circuit constructed in such a way that three distinguishable states should emerge, characterized by the expression and repression of two different reporter fluorescent proteins.
Potential Risks
Given its natural habitat of the large bowel of mammals, E. coli will not likely survive for long periods in soil, water, or air. E. coli K-12 has been shown to have poorer survival characteristics in soil and water than other E. coli . The ability of E.coli to survive under environmental conditionsis thus very limited.
Genetic Modifications
The project “Thermocoli” consists in a circuit of transcription factors and reporter genes, some of which are under the post-transcriptional regulation of thermoregulable RNA elements, also known as RNA thermometers. These genes are arrange in a circuit constructed in such a way that three distinguishable states should emerge, characterized by the expression and repression of two different reporter fluorescent proteins.
Genetic Modifications
The project “Thermocoli” consists in a circuit of transcription factors and reporter genes, some of which are under the post-transcriptional regulation of thermoregulable RNA elements, also known as RNA thermometers. These genes are arrange in a circuit constructed in such a way that three distinguishable states should emerge, characterized by the expression and repression of two different reporter fluorescent proteins.
Genetic Modifications
The project “Thermocoli” consists in a circuit of transcription factors and reporter genes, some of which are under the post-transcriptional regulation of thermoregulable RNA elements, also known as RNA thermometers. These genes are arrange in a circuit constructed in such a way that three distinguishable states should emerge, characterized by the expression and repression of two different reporter fluorescent proteins.
Risk Assessment:Download here
