Team:Marburg/Notebook:October
From 2013.igem.org
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<li>Detect with 1 ml/blot Clarity Western ECL solution (Biorad)</li> | <li>Detect with 1 ml/blot Clarity Western ECL solution (Biorad)</li> | ||
</ul> | </ul> | ||
+ | |||
+ | <table class="gel pcr"> | ||
+ | <colgroup> | ||
+ | <col width="50%" /> | ||
+ | <col width="50%" /> | ||
+ | </colgroup> | ||
+ | <thead> | ||
+ | <tr> | ||
+ | <th colspan="2" class="title">SDS gel</th> | ||
+ | </tr> | ||
+ | </thead> | ||
+ | <tbody> | ||
+ | <tr> | ||
+ | <td> | ||
+ | <img src="https://static.igem.org/mediawiki/2013/5/58/Mr-western-2410.png" width="100%" alt="wester-blot-image" /> | ||
+ | </td> | ||
+ | <td> | ||
+ | <p> | ||
+ | <span class="gel-elc">Gel substances</span> | ||
+ | <ul class="gel-sub"> | ||
+ | <li>12 % Acryl amide gel</li> | ||
+ | <li>6 µl PageRuler Prestained Protein Ladder (Thermo Scientific)</li> | ||
+ | </p> | ||
+ | |||
+ | |||
+ | <table class="gel"> | ||
+ | <colgroup> | ||
+ | <col width="9%" /> | ||
+ | <col width="2%" /> | ||
+ | <col width="42%" /> | ||
+ | <col width="2%" /> | ||
+ | <col width="45%" /> | ||
+ | </colgroup> | ||
+ | <tr> | ||
+ | <td><b>Upper gel</b></td> | ||
+ | </tr> | ||
+ | <thead> | ||
+ | <th>Lane</th> | ||
+ | <th> </th> | ||
+ | <th>Content</th> | ||
+ | <th> </th> | ||
+ | <th>Expectations</th> | ||
+ | </thead> | ||
+ | <tr> | ||
+ | <td>2</td> | ||
+ | <th> </th> | ||
+ | <td>Darkness</td> | ||
+ | <th> </th> | ||
+ | <td>26 kDa (lc) + 55 kDa (hc)</td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td>3</td> | ||
+ | <th> </th> | ||
+ | <td>Ball mill</td> | ||
+ | <th> </th> | ||
+ | <td>26 kDa (lc) + 55 kDa (hc)</td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td>4</td> | ||
+ | <th> </th> | ||
+ | <td>Glass beads + prot. inh.</td> | ||
+ | <th> </th> | ||
+ | <td>26 kDa (lc) + 55 kDa (hc)</td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td>5</td> | ||
+ | <th> </th> | ||
+ | <td>Glass beads</td> | ||
+ | <th> </th> | ||
+ | <td>26 kDa (lc) + 55 kDa (hc)</td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td>6</td> | ||
+ | <th> </th> | ||
+ | <td>Sonication (total)</td> | ||
+ | <th> </th> | ||
+ | <td>26 kDa (lc) + 55 kDa (hc)</td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td>7</td> | ||
+ | <th> </th> | ||
+ | <td>Sonication (SN)</td> | ||
+ | <th> </th> | ||
+ | <td>26 kDa (lc) + 55 kDa (hc)</td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td>8</td> | ||
+ | <th> </th> | ||
+ | <td>Sonication (P)</td> | ||
+ | <th> </th> | ||
+ | <td>26 kDa (lc) + 55 kDa (hc)</td> | ||
+ | </tr> | ||
+ | </table> | ||
+ | </td> | ||
+ | </tr> | ||
+ | </tbody> | ||
+ | </table> | ||
+ | |||
+ | <p>Result: The highest amounts of proteins were gained using sonication and the ball mill.</p> | ||
</div> | </div> |
Revision as of 19:02, 28 October 2013