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Team:ETH Zurich/Notebook
From 2013.igem.org
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<div style="font-size:18px;font-family:Verdana;position:static;margin-top:10px"> <b>Kick off event</b> 19.06.13 </div> | <div style="font-size:18px;font-family:Verdana;position:static;margin-top:10px"> <b>Kick off event</b> 19.06.13 </div> | ||
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| - | <div style="font-size:18px;font-family:Verdana;position:static"> <b>Brainstorming</b> 19.06.13 - 3.07.13 </div> | + | <div style="font-size:18px;font-family:Verdana;position:static"> <b>Brainstorming</b> 19.06.13 - 3.07.13 </div><br> |
| + | <div style="color:white;font-size:18px;font-family:Verdana;position:static"> Coming up with different ideas for our iGEM project, considering the advantages and disadvantages and the final impact. In the end, we decided on something innovative and fun!</div> | ||
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<div style="font-size:18px;font-family:Verdana;position:static"> <b>Start Work</b> 4.07.13 </div> | <div style="font-size:18px;font-family:Verdana;position:static"> <b>Start Work</b> 4.07.13 </div> | ||
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| - | <div style="color:white;font-size:18px;font-family:Verdana;position:static"> <b>Week 1</b> : Planning of responsibilities | + | <div style="color:white;font-size:18px;font-family:Verdana;position:static"> <b>Week 1</b> : PLANNING AND LAB WORK. Planning of responsibilities and establishment of roles of all team members; starting the wiki; gathering more information on the project by doing research in literature; designing a logo for our product, coming up with a catchy slogan, and deciding on a name for the final project; designing initial experiments and learning many necessary lab techniques (some of the first lab tasks included making buffers, media, chemical competent cells, choosing biobricks for our cells, and doing transformations of the first chosen bricks. </div> |
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| - | <div style="color:white;font-size:18px;font-family:Verdana;position:static"> <b>Week 2</b> : | + | <div style="color:white;font-size:18px;font-family:Verdana;position:static"> <b>Week 2</b> : CONTINUATION OF LAB WORK AND BEGINNING OF MODELING. Transforming all the bricks and clonning to built our pathways. Decide about 4 hydrolases : NagZ, PhoA, GusA and Aes and the according substrates to color them. We encouter some difficulties during transformiation of triple knockout cells with the hydrolases</div> |
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| - | <div style="color:white;font-size:18px;font-family:Verdana;position:static"> <b>Week 3</b> : All transformation and clonning is done.</div> | + | <div style="color:white;font-size:18px;font-family:Verdana;position:static"> <b>Week 3 - 4</b> : MORE LAB WORK AND MODELING All transformation and clonning is done. Start of AHL diffusion experiments for characterization of the AHL diffusion. The receiver cells with GFP are done and work well on plates an liquid culture. Primer design for the mutation of LuxR promoter to alter sensitvity.</div> |
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| - | <div style="color:white;font-size:18px;font-family:Verdana;position:static"> <b>Week | + | <div style="color:white;font-size:18px;font-family:Verdana;position:static"> <b>Week 5 - 8</b> : LAB WORK, MODELING, UPDATING WIKI. Working on AHL diffusion, transform an other strain with T7 polymerase to test the substrates. Working on transformation of the triple knockout cells. Retransform LuxI construct because the first transformation din't have an RBS.</div> |
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| - | <div style="color:white;font-size:18px;font-family:Verdana;position:static"> <b>Week | + | <div style="color:white;font-size:18px;font-family:Verdana;position:static"> <b>Week 6</b> : LAB WORK AND WIKI. Working on AHL diffusion, transform an other strain with T7 polymerase to test the substrates. Working on transformation of the triple knockout cells. Retransform LuxI construct because the first transformation din't have an RBS.</div> |
Revision as of 14:11, 18 August 2013
Kick off event 19.06.13
Brainstorming 19.06.13 - 3.07.13
Coming up with different ideas for our iGEM project, considering the advantages and disadvantages and the final impact. In the end, we decided on something innovative and fun!
Start Work 4.07.13
Week 1 : PLANNING AND LAB WORK. Planning of responsibilities and establishment of roles of all team members; starting the wiki; gathering more information on the project by doing research in literature; designing a logo for our product, coming up with a catchy slogan, and deciding on a name for the final project; designing initial experiments and learning many necessary lab techniques (some of the first lab tasks included making buffers, media, chemical competent cells, choosing biobricks for our cells, and doing transformations of the first chosen bricks.
Week 2 : CONTINUATION OF LAB WORK AND BEGINNING OF MODELING. Transforming all the bricks and clonning to built our pathways. Decide about 4 hydrolases : NagZ, PhoA, GusA and Aes and the according substrates to color them. We encouter some difficulties during transformiation of triple knockout cells with the hydrolases
Week 3 - 4 : MORE LAB WORK AND MODELING All transformation and clonning is done. Start of AHL diffusion experiments for characterization of the AHL diffusion. The receiver cells with GFP are done and work well on plates an liquid culture. Primer design for the mutation of LuxR promoter to alter sensitvity.
Week 5 - 8 : LAB WORK, MODELING, UPDATING WIKI. Working on AHL diffusion, transform an other strain with T7 polymerase to test the substrates. Working on transformation of the triple knockout cells. Retransform LuxI construct because the first transformation din't have an RBS.
Week 6 : LAB WORK AND WIKI. Working on AHL diffusion, transform an other strain with T7 polymerase to test the substrates. Working on transformation of the triple knockout cells. Retransform LuxI construct because the first transformation din't have an RBS.
