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Team:Paris Saclay/Notebook/July/10
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='''Notebook : July 10'''= | ='''Notebook : July 10'''= | ||
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| + | ==''Summary:''== | ||
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| + | For regulation system: | ||
| + | *prepared the solution of BioBrick fnr repressor in PsB1C3 plasmid for DNA sequencing | ||
| + | *the terminator transformation of yesterday did not work, a second transformation had | ||
| + | been done for it | ||
| + | *The transformation for RBS+LacZ+terminator plasmid into competent cells was | ||
| + | performed. | ||
| + | *after the transformation PSB3K3 plasmid in competent cells, these cells were cultured in a | ||
| + | liquid nutritive medium | ||
| + | For PSBs sensor system: | ||
| + | *the ligation products were transformed into competent cells and were cultured on solid | ||
| + | medium with their specific antibiotics | ||
| + | |||
=='''Lab work'''== | =='''Lab work'''== | ||
Revision as of 03:00, 28 August 2013
Notebook : July 10
Summary:
For regulation system:
- prepared the solution of BioBrick fnr repressor in PsB1C3 plasmid for DNA sequencing
- the terminator transformation of yesterday did not work, a second transformation had
been done for it
- The transformation for RBS+LacZ+terminator plasmid into competent cells was
performed.
- after the transformation PSB3K3 plasmid in competent cells, these cells were cultured in a
liquid nutritive medium For PSBs sensor system:
- the ligation products were transformed into competent cells and were cultured on solid
medium with their specific antibiotics
Lab work
constructing
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