What about those hydrolases ?

We use hydrolases to realize a colorimetric response by conversion of a specific substrate. We will use five hydrolases : Beta-Galactosidase (lacZ),Beta-Glucuronidase (GusA); alkaline phosphatase (phoA);Glycoside hydrolase (NagZ) and the acetyl esterase (aes).

On this page you will find all relevant information on the hydrolases and enzyme substrate reactions based on papers and our results.

What about the hydolases? How do they work and where do they come from? Why do we use hydrolases?

Colorimetric response in liquid culture
Colorimetric response on LB-Agar

Beta-galactosidase (LacZ)

Fig.5: X-Gal on LacZ expressing colony

Fig.6: Beta-green-X-gal on LacZ expressing colony

Alkaline phosphatase(PhoA)

Fig.2: NPP on PhoA expressing colony

Acetyl esterase (Aes)

Fig.1: Magenta butyrate on Aes expressing colony

Glycoside hydrolase (NagZ)

Fig.4: X-Glucnac on NagZ expressing colony

Beta-glucuronidase (GusA)

Fig.3 Magenta glucuronide on GusA expressing colony