Team:Calgary/Sandbox/Notebook/Protocols
From 2013.igem.org
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<li><a href="https://2013.igem.org/Team:Calgary/Sandbox/Notebook/Protocols/BacterialTransformation" target="_blank">Bacterial Transformation</a></li> | <li><a href="https://2013.igem.org/Team:Calgary/Sandbox/Notebook/Protocols/BacterialTransformation" target="_blank">Bacterial Transformation</a></li> | ||
<li><a href="https://2013.igem.org/Team:Calgary/Sandbox/Notebook/Protocols/BradfordAssay" target="_blank">Bradford Assay</a></li> | <li><a href="https://2013.igem.org/Team:Calgary/Sandbox/Notebook/Protocols/BradfordAssay" target="_blank">Bradford Assay</a></li> | ||
- | <li><a href="https://2013.igem.org/Team:Calgary/Sandbox/Notebook/Protocols/ | + | <li><a href="https://2013.igem.org/Team:Calgary/Sandbox/Notebook/Protocols/FreezeThawProtocolforProteinSamples" target="_blank">Freeze-Thaw Protocol for Protein Samples</a></li> |
<li><a href="https://2013.igem.org/Team:Calgary/Sandbox/Notebook/Protocols/GelExtraction" target="_blank">Gel Extraction</a></li> | <li><a href="https://2013.igem.org/Team:Calgary/Sandbox/Notebook/Protocols/GelExtraction" target="_blank">Gel Extraction</a></li> | ||
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<li><a href="https://2013.igem.org/Team:Calgary/Sandbox/Notebook/Protocols/GlassBeadsCellLysisProtocolforProteinSamples" target="_blank">Glass Beads Cell Lysis Protocol for Protein Samples</a></li> | <li><a href="https://2013.igem.org/Team:Calgary/Sandbox/Notebook/Protocols/GlassBeadsCellLysisProtocolforProteinSamples" target="_blank">Glass Beads Cell Lysis Protocol for Protein Samples</a></li> | ||
+ | <li><a href="https://2013.igem.org/Team:Calgary/Sandbox/Notebook/Protocols/GlycerolStocks" target="_blank">Glycerol Stocks</a></li> | ||
<li><a href="https://2013.igem.org/Team:Calgary/Sandbox/Notebook/Protocols/LBAgarPlates" target="_blank">LB Agar Plates</a></li> | <li><a href="https://2013.igem.org/Team:Calgary/Sandbox/Notebook/Protocols/LBAgarPlates" target="_blank">LB Agar Plates</a></li> | ||
+ | <li><a href="https://2013.igem.org/Team:Calgary/Sandbox/Notebook/Protocols/MakingChemicallyCompetentEcoliCells" target="_blank">Making Chemically Competent <i>E. coli</i> Cells</a></li> | ||
<li><a href="https://2013.igem.org/Team:Calgary/Sandbox/Notebook/Protocols/OvernightCultures" target="_blank">Overnight Cultures</a></li> | <li><a href="https://2013.igem.org/Team:Calgary/Sandbox/Notebook/Protocols/OvernightCultures" target="_blank">Overnight Cultures</a></li> | ||
<li><a href="https://2013.igem.org/Team:Calgary/Sandbox/Notebook/Protocols/PCRPurification" target="_blank">PCR Purification</a></li> | <li><a href="https://2013.igem.org/Team:Calgary/Sandbox/Notebook/Protocols/PCRPurification" target="_blank">PCR Purification</a></li> | ||
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<li><a href="https://2013.igem.org/Team:Calgary/Sandbox/Notebook/Protocols/VerificationDigest" target="_blank">Verification Digest</a></li> | <li><a href="https://2013.igem.org/Team:Calgary/Sandbox/Notebook/Protocols/VerificationDigest" target="_blank">Verification Digest</a></li> | ||
</ul> | </ul> | ||
- | <h2> | + | <h2>TALEs</h2> |
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- | <li><a href=" | + | <li><a href="https://2013.igem.org/Team:Calgary/Sandbox/Notebook/Protocols/GelShiftAssay" target="_blank">Gel Shift Assay</a></li> |
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</html> | </html> |
Revision as of 06:05, 18 September 2013
Protocols
General Protocols
- Agarose Gel Electrophoresis
- Bacterial Transformation
- Bradford Assay
- Freeze-Thaw Protocol for Protein Samples
- Gel Extraction
- Glass Beads Cell Lysis Protocol for Protein Samples
- Glycerol Stocks
- LB Agar Plates
- Making Chemically Competent E. coli Cells
- Overnight Cultures
- PCR Purification
- Plasmid Purification from E. coli
- Rehydration of Registry DNA
- Restriction Digestion, Antarctic Phosphatase, and Ligation
- SDS PAGE Gel
- Verification Digest