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Team:Paris Saclay/Notebook/July/9
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{{Team:Paris_Saclay/incl_debut_generique}} | {{Team:Paris_Saclay/incl_debut_generique}} | ||
='''Notebook : July 9'''= | ='''Notebook : July 9'''= | ||
| + | |||
| + | ==='''Summary :'''=== | ||
| + | For regulation system : | ||
| + | *extraction of BioBrick BBa_K1155000 in concentrated medium for further DNA sequencing. | ||
| + | For the construction of BioBrick | ||
| + | *the terminator BBa_B0010 in PSB1A2 plasmid was transformed into competent cells and then was cultured on solid medium for confirmation | ||
| + | *the plasmid PSB3K3 was transformed into competent cells and then was cultured on solid medium for confirmation | ||
| + | For sensor system: | ||
| + | *A series of digestion, ligation were performed for BioBrick BphR2, BphR1, BphA1 | ||
=='''Lab work'''== | =='''Lab work'''== | ||
Revision as of 09:12, 21 September 2013
Notebook : July 9
Summary :
For regulation system :
- extraction of BioBrick BBa_K1155000 in concentrated medium for further DNA sequencing.
For the construction of BioBrick
- the terminator BBa_B0010 in PSB1A2 plasmid was transformed into competent cells and then was cultured on solid medium for confirmation
- the plasmid PSB3K3 was transformed into competent cells and then was cultured on solid medium for confirmation
For sensor system:
- A series of digestion, ligation were performed for BioBrick BphR2, BphR1, BphA1
Lab work
constructing
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