Team:Marburg/Notebook:June

From 2013.igem.org

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<fieldset class="experiment digest">
<fieldset class="experiment digest">
     <legend><a name="dig">Digest</a></legend>
     <legend><a name="dig">Digest</a></legend>
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<div class="exp-content">
<div class="exp-content">
<p><ul class="digest">
<p><ul class="digest">
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<li>1 µl Plasmid (pSB1C3-iBB39,pSB1A3-iBB49 or pSB1C3-iBB6315)</li>
+
<li>1 µl Plasmid (pSB1C3-iBB39,pSB1A3-iBB49 and pSB1C3-iBB6315)</li>
<li>0.3 µl EcoRI</li>
<li>0.3 µl EcoRI</li>
<li>0.3 µl PstI</li>
<li>0.3 µl PstI</li>
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</fieldset>
</fieldset>
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<div class="notebooky-entry">
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<h2 class="title">
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<a name="26-06-2013">26.06.2013</a>
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</h2>
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<fieldset class="experiment digest">
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    <legend><a name="dig">Digest</a></legend>
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    <div class="investigator">
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<span class="inv">Investigator:</span>
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<span class="inv-names">Dominik</span>
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</div>
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    <div class="aim">
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<span class="aim">Aim:</span>
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<span class="aim-desc">Construction of the plasmids pSB1A3-iBB10+9, pSB1A3-iBB11+9, pSB1A3-iBB12+9 and pSB1A3-iBB13+9</span>
 +
</div>
 +
<div class="exp-content">
 +
<p><ul class="digest">
 +
<li>1 µl Plasmid (pSB1C3-iBB10, pSB1C3-iBB11, pSB1C3-iBB12 and pSB1C3-iBB13)</li>
 +
<li>0.5 µl EcoRI</li>
 +
<li>0.5 µl SpeI</li>
 +
<li>2 µl CutSmart</li>
 +
<li>12 µl ddH2O</li>
 +
</ul>
 +
 +
<p><ul class="digest">
 +
<li>1 µl Plasmid (pSB1C3-iBB9)</li>
 +
<li>0.5 µl XbaI</li>
 +
<li>0.5 µl PstI</li>
 +
<li>2 µl CutSmart</li>
 +
<li>12 µl ddH2O</li>
 +
</ul>
 +
 +
<p><ul class="digest">
 +
<li>1 µl Plasmid (pSB1A3)</li>
 +
<li>0.5 µl EcoRI</li>
 +
<li>0.5 µl PstI</li>
 +
<li>2 µl CutSmart</li>
 +
<li>12 µl ddH2O</li>
 +
</ul>
 +
 +
<p>The samples were incubated for 1.5 h at 37° C.</p>
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<table class="gel digest">
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<colgroup>
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<col width="50%" />
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<col width="50%" />
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</colgroup>
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<thead>
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<tr>
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<th colspan="2" class="title">Gel electrophoresis</th>
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</tr>
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</thead>
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<tbody>
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<tr>
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<td>
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<img src="beispiel.png" width="50%" alt="gel-electrophoresis-image" />
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</td>
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<td>
 +
<p>
 +
<span class="gel-elc">Gel substances</span>
 +
<ul class="gel-sub">
 +
<li>1% Agarose gel</li>
 +
<li>10 µl RedSafe in 50 ml gel</li>
 +
<li>x µl Hyper Ladder</li>
 +
</ul>
 +
</p>
 +
<p>
 +
<span class="exp">Expactations</span>
 +
<ul class="exp">
 +
<li>Lane 1: 5300 kbp</li>
 +
<li>Lane 2: 3300 kbp</li>
 +
<li>Lane 3: 1300 kbp</li>
 +
</ul>
 +
</p>
 +
<p>All expected fragments were present. The relevant fragments were cut from the gel and then purified via “Qiagen Gel Extraction Kit” (Qiagen, Düsseldorf).</p>
 +
</td>
 +
</tr>
 +
</tbody>
 +
</table>
 +
</div>
 +
</fieldset>

Revision as of 21:51, 21 September 2013

Notebook

25.06.2013

Digest
Investigator: Dominik
Aim: Test digest of pSB1A3-iBB39, pSB1A3-iBB49 and pSB1C3-iBB6315 with EcoRI and PstI

  • 1 µl Plasmid (pSB1C3-iBB39,pSB1A3-iBB49 and pSB1C3-iBB6315)
  • 0.3 µl EcoRI
  • 0.3 µl PstI
  • 1 µl CutSmart
  • 7.4 µl ddH2O

The samples were incubated for 1 h at 37° C.

Gel electrophoresis
gel-electrophoresis-image

Gel substances

  • 1% Agarose gel
  • 10 µl RedSafe in 50 ml gel
  • x µl Hyper Ladder

Expactations

  • Lane 1: 5300 kbp
  • Lane 2: 3300 kbp
  • Lane 3: 1300 kbp

Worked as expected.

26.06.2013

Digest
Investigator: Dominik
Aim: Construction of the plasmids pSB1A3-iBB10+9, pSB1A3-iBB11+9, pSB1A3-iBB12+9 and pSB1A3-iBB13+9

  • 1 µl Plasmid (pSB1C3-iBB10, pSB1C3-iBB11, pSB1C3-iBB12 and pSB1C3-iBB13)
  • 0.5 µl EcoRI
  • 0.5 µl SpeI
  • 2 µl CutSmart
  • 12 µl ddH2O

  • 1 µl Plasmid (pSB1C3-iBB9)
  • 0.5 µl XbaI
  • 0.5 µl PstI
  • 2 µl CutSmart
  • 12 µl ddH2O

  • 1 µl Plasmid (pSB1A3)
  • 0.5 µl EcoRI
  • 0.5 µl PstI
  • 2 µl CutSmart
  • 12 µl ddH2O

The samples were incubated for 1.5 h at 37° C.

Gel electrophoresis
gel-electrophoresis-image

Gel substances

  • 1% Agarose gel
  • 10 µl RedSafe in 50 ml gel
  • x µl Hyper Ladder

Expactations

  • Lane 1: 5300 kbp
  • Lane 2: 3300 kbp
  • Lane 3: 1300 kbp

All expected fragments were present. The relevant fragments were cut from the gel and then purified via “Qiagen Gel Extraction Kit” (Qiagen, Düsseldorf).

Retrieved from "http://2013.igem.org/Team:Marburg/Notebook:June"