Team:Calgary/Sandbox/Notebook/Protocols
From 2013.igem.org
(Difference between revisions)
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<li><a href="https://2013.igem.org/Team:Calgary/Sandbox/Notebook/Protocols/FreezeThawProtocolforProteinSamples" target="_blank">Freeze-Thaw Protocol for Protein Samples</a></li> | <li><a href="https://2013.igem.org/Team:Calgary/Sandbox/Notebook/Protocols/FreezeThawProtocolforProteinSamples" target="_blank">Freeze-Thaw Protocol for Protein Samples</a></li> | ||
<li><a href="https://2013.igem.org/Team:Calgary/Sandbox/Notebook/Protocols/GlassBeadsCellLysisProtocolforProteinSamples" target="_blank">Glass Beads Cell Lysis Protocol for Protein Samples</a></li> | <li><a href="https://2013.igem.org/Team:Calgary/Sandbox/Notebook/Protocols/GlassBeadsCellLysisProtocolforProteinSamples" target="_blank">Glass Beads Cell Lysis Protocol for Protein Samples</a></li> | ||
+ | <li><a href="https://2013.igem.org/Team:Calgary/Sandbox/Notebook/Protocols/OsmoticShock" target="_blank">Osmotic Shock</a></li> | ||
<li><a href="https://2013.igem.org/Team:Calgary/Sandbox/Notebook/Protocols/SDSPAGEGel" target="_blank">SDS PAGE Gel</a></li> | <li><a href="https://2013.igem.org/Team:Calgary/Sandbox/Notebook/Protocols/SDSPAGEGel" target="_blank">SDS PAGE Gel</a></li> | ||
+ | <li><a href="https://2013.igem.org/Team:Calgary/Sandbox/Notebook/Protocols/WesternBlot" target="_blank">Western Blot</a></li> | ||
</ul> | </ul> | ||
<h2>TALE Protocols</h2> | <h2>TALE Protocols</h2> | ||
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<li><a href="https://2013.igem.org/Team:Calgary/Sandbox/Notebook/Protocols/GelShiftAssay" target="_blank">Gel Shift Assay</a></li> | <li><a href="https://2013.igem.org/Team:Calgary/Sandbox/Notebook/Protocols/GelShiftAssay" target="_blank">Gel Shift Assay</a></li> | ||
</ul> | </ul> | ||
- | <h2> | + | <h2>Ferritin Protocols</h2> |
<ul> | <ul> | ||
- | <li> | + | <li><a href="https://2013.igem.org/Team:Calgary/Sandbox/Notebook/Protocols/Kinetics" target="_blank">Kinetics</a></li> |
</ul> | </ul> | ||
</section> | </section> | ||
</html> | </html> |
Revision as of 22:19, 22 September 2013
Protocols
General Protocols
- Bacterial Transformation
- Glycerol Stocks
- LB Agar Plates
- Making Chemically Competent E. coli Cells
- Overnight Cultures
DNA Protocols
- Agarose Gel Electrophoresis
- Colony PCR
- Gel Extraction
- Kapa PCR
- PCR Purification
- Plasmid Purification from E. coli
- Rehydration of Registry DNA
- Restriction Digestion, Antarctic Phosphatase, and Ligation
- Verification Digest
Protein Protocols
- Bradford Assay
- Freeze-Thaw Protocol for Protein Samples
- Glass Beads Cell Lysis Protocol for Protein Samples
- Osmotic Shock
- SDS PAGE Gel
- Western Blot