"
Team:ETH Zurich/Templates/Test
From 2013.igem.org
(Difference between revisions)
| Line 8: | Line 8: | ||
<html> | <html> | ||
| - | |||
| - | |||
<img src="https://static.igem.org/mediawiki/2013/thumb/b/b7/Plasmidmap.png/800px-Plasmidmap.png" usemap="#map3" id="imagemap3"> | <img src="https://static.igem.org/mediawiki/2013/thumb/b/b7/Plasmidmap.png/800px-Plasmidmap.png" usemap="#map3" id="imagemap3"> | ||
| - | <map id="map3" name="map3"><area shape="rect" alt="" title="NagZ" coords="259,77,340,114" href="" target=""/><area shape="poly" alt="" title="" coords="161,86,215,82,215,70,244,98,216,123,216,109,158,108" href="" target="" /><area shape="rect" alt="" title="" coords="255,230,327,263" href="" target="" /><area shape="poly" alt="" title="" coords="157,245,211,240,210,231,239,258,216,277,217,266,152,266" href="" target="" /><area shape="rect" alt="" title="" coords="682,76,763,113" href="" target="" /><area shape="poly" alt="" title="" coords="591,105,652,95,648,81,676,104,654,132,651,116,594,125" href="" target="" /><area shape="poly" alt="" title="" coords="782,85,841,88,843,74,863,102,839,124,839,110,780,107" href="" target="" /><area shape="poly" alt="" title="" coords="881,85,965,99,960,131,875,119" href="" target="" /><area shape="poly" alt="" title="" coords="581,263,642,254,637,238,664,264,643,292,640,275,581,287" href="" target="" /><area shape="rect" alt="" title="" coords="675,237,738,272" href="" target="" /><area shape="rect" alt="" title="" coords="872,244,951,277" href="" target="" /><area shape="poly" alt="" title="" coords="768,246,828,247,827,230,853,257,828,280,828,267,767,270" href="" target="" /> | + | <map id="map3" name="map3"><area shape="rect" alt="" title="NagZ hydrolase BBa_K1216003" coords="259,77,340,114" href="" target=""/><area shape="poly" alt="" title="constitutive promoter BBa_J23100 " coords="161,86,215,82,215,70,244,98,216,123,216,109,158,108" href="" target="" /><area shape="rect" alt="" title="" coords="255,230,327,263" href="" target="" /><area shape="poly" alt="" title="" coords="157,245,211,240,210,231,239,258,216,277,217,266,152,266" href="" target="" /><area shape="rect" alt="" title="" coords="682,76,763,113" href="" target="" /><area shape="poly" alt="" title="" coords="591,105,652,95,648,81,676,104,654,132,651,116,594,125" href="" target="" /><area shape="poly" alt="" title="" coords="782,85,841,88,843,74,863,102,839,124,839,110,780,107" href="" target="" /><area shape="poly" alt="" title="" coords="881,85,965,99,960,131,875,119" href="" target="" /><area shape="poly" alt="" title="" coords="581,263,642,254,637,238,664,264,643,292,640,275,581,287" href="" target="" /><area shape="rect" alt="" title="" coords="675,237,738,272" href="" target="" /><area shape="rect" alt="" title="" coords="872,244,951,277" href="" target="" /><area shape="poly" alt="" title="" coords="768,246,828,247,827,230,853,257,828,280,828,267,767,270" href="" target="" /> |
<!-- Created by Online Image Map Editor (http://www.maschek.hu/imagemap/index) --></map> | <!-- Created by Online Image Map Editor (http://www.maschek.hu/imagemap/index) --></map> | ||
| Line 18: | Line 16: | ||
<script type="text/javascript"> | <script type="text/javascript"> | ||
$(document).ready(function(){ | $(document).ready(function(){ | ||
| - | $('# | + | $('#imagemap3').mapster({ |
fillColor: 'c2d8f1', | fillColor: 'c2d8f1', | ||
fillOpacity: 0.6, /* | fillOpacity: 0.6, /* | ||
| Line 28: | Line 26: | ||
}); | }); | ||
}); | }); | ||
| - | |||
| - | |||
| - | |||
</html> | </html> | ||
Revision as of 16:37, 23 September 2013
Final Circuit
For the final Colisweeper circuit we plan a four plasmid system. The mine cells constitutively express LuxI for signal generation and NagZ as identifier hydrolase. In the non-mine cells LuxR is expressed constitutively to process the OHHL signal. PhoA is expressed constitutively as well as reporter for safe cells. Aes and GusA are expressed from pLux promoters with different sensitivities. You can find all the biobricks we used and our own new biobricks in the figure below.
