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Team:Paris Saclay/Notebook/July/10
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===='''Objective : obtaining biobricks in PSB3K3'''==== | ===='''Objective : obtaining biobricks in PSB3K3'''==== | ||
| - | ====''' | + | ===='''1 - Mini and maxi preparation of the culture of Bba_J04450'''==== |
Abdou | Abdou | ||
Revision as of 19:41, 26 September 2013
Contents |
Notebook : July 10
Lab work
A - Aerobic/Anaerobic regulation system
Objective : obtaining Bba_K1155003, Bba_K1155007
1 - Transformation of Bba_I732019 in DH5α
Sheng
Protocol : Bacterial transformation
Objective : obtaining biobricks in PSB3K3
1 - Mini and maxi preparation of the culture of Bba_J04450
Abdou
|
Transformation of Bba_J044500 of 07/09/13 didn't work. We obtain 34 colonies. We will extract DNA by doing mini and maxi preparations. |
Protocol : Mini and maxi preparation
We let our preparations at 37°C, 200rpm with 5mL of LB and 5mL of kanamicine antibiotic.
B - PCB sensor system
Objective : obtaining Bba_K1155001, Bba_K1155002, BphR2 protein
1 - Transformation of ligation of PSB1C3 and BphA1 or BphR1 or BphR2
Abdou
Protocol : Bacterial transformation
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