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| - | <li class="active"><a href="#Introductory">New UANL-iGemers</a></li>
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| - | <li><a href="#CIDEB prep">iGEM _CIDEB2013</a>i</li>
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| - | <li><a href="#Project">Project circuit</a></li>
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| - | <li><a href="#Types_of_RNAT">Types of RNAT</a></li>
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| - | <p><h3>Training Workshop iGEM _CIDEB2013</h3><hr></p></div>
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| - | <p>iGEM-UANL team conducted a training workshop to enlist the team <a href="http://2013HS.igem.org/Team:CIDEB-UANL_Mexico" target="_blank">CIDEB-UANL Mexico</a> to the iGEM competition for its participation in <a href="https://igem.org/Results?year=2013&division=high_school" target="_blank">iGEM HSD 2013</a>. They did fabulous! They came 2nd runner up in the competition just like the last year</p><br>
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| - | <p>The topics that were included in the workshop were about molecular biology related with the nucleic acids function and the proteins involved in the “Central dogma”. About the laboratory, different techniques of molecular biology in the laboratory that are used in the genetic engineering and the synthetic biology, were taught to the students and a Practices Handbook was given to them elaborated by the instructors of the workshop.</p><br>
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| - | <p><br><h2><b>Description</b><a href="#" class="btn btn-info"><font color="#fff">Back to top</font></a></h2><br></p>
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| - | <p><br><b>Objectives</b><br></p>
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| - | <p><b>•</b> To prepare the high school students from the CIDEB that are interested in participate in iGEM in the High School Division (HSD) of mentioned workshop in its version 2013 with introductory topics to molecular biology, engineering, nucleic acids manipulation, biology with data processing and synthetic biology.</p>
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| - | <p><b>•</b> To present to the interested students in an easy, clear and precise panorama about the principal laboratory techniques that are used in synthetic biology in order to make them develop the ability to work in the laboratory.</p>
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| - | <p><b>•</b> To aid the students with the doubts that can be presented during the workshop in order to increase their interest in molecular and synthetic biology.</p>
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| - | <p><br><b>Activities</b><br></p>
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| - | <p>During the Training workshop week, some activities took place:</p>
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| - | <p><b>1.</b> In the semester July - December 2012, during 8 Saturdays some theory classes were given to the students with an approximate duration of 2 hours.</p>
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| - | <p><b>2.</b> The laboratory workshop was given during the first week of December. Before starting work with the laboratory practice, an explanation was given. </p>
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| - | <p><b>3.</b> The participants were divided in groups and there was designed a instructor for each group to help them with some doubts they can have so they were able to perform the practice by themselves.</p>
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| - | <p><br><b>The TOPICS in the Training Workshop Theory Class were the following:</b><br></p>
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| - | <p><b>Day 1</b> – 29 September</p>
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| - | <p><b>-</b> What is DNA?</p>
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| - | <p><b>-</b> DNA Properties</p>
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| - | <p><b>-</b> DNA Structure</p>
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| - | <p><b>Day 2</b> – 6 October</p>
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| - | <p><b>-</b> Genomics</p>
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| - | <p><b>-</b> Structural composition of a gene</p>
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| - | <p><b>-</b> Gene Function</p>
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| - | <p><b>Day 3</b> – 13 October </p>
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| - | <p><b>-</b> Proteins</p>
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| - | <p><b>-</b> Function of proteins</p>
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| - | <p><b>-</b> Types of Proteins</p>
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| - | <p><b>Day 4</b> – 20 October </p>
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| - | <p><b>-</b> Genetics Egineering</p>
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| - | <p><b>-</b> Characterization through restriction enzymes</p>
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| - | <p><b>Day 5</b> – 27 October </p>
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| - | <p><b>-</b> Synthetic Biology</p>
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| - | <p><b>-</b> Assembly using Building Standards</p>
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| - | <p><b>Day 6</b> – 3 November </p>
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| - | <p><b>-</b> BioBricks™ in the Parts Registry</p>
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| - | <p><b>-</b> A detailed view to the BioBricks™</p>
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| - | <p><b>Day 7</b> – 10 November </p>
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| - | <p><b>-</b> Biology Systems</p>
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| - | <p><b>-</b> Metabolic Engineering</p>
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| - | <p><b>Day 8</b> – 24 November </p>
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| - | <p><b>-</b> Checking of the iGEM projects</p>
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| - | <p><b>-</b> Project requirements:</p>
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| - | <p><b>• </b> Development of ideas</p>
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| - | <p><b>• </b> Parts construction</p>
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| - | <p><b>• </b> Human Practices</p>
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| - | <p><b>• </b> Poster</p>
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| - | <p><b>• </b> Wiki</p>
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| - | <p><b>• </b> Presentation</p>
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| - | <p> Then, we continued with the practice section of the workshop as the following:</p>
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| - | <p><b>Day 1</b> – 10 December</p>
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| - | <p><b>• Practice 1:</b> Manipulation of DNA plates lyophilized of BioBrick™</p>
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| - | <p><b>• Practice 2:</b> Preparation and transformation of calcium competent bacteria</p>
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| - | <p><b>Day 2</b> – 11 December</p>
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| - | <p><b>• Practice 3:</b> Inoculation and cultivation</p>
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| - | <p><b>• Practice 4:</b> Miniprep</p>
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| - | <p><b>• Practice 5:</b> Quantification by spectrophotometry</p>
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| - | <p><b>Day 2</b> – 12 December </p>
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| - | <p><b>• Practice 6:</b> Characterization through restriction enzymes</p>
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| - | <p><b>Day 4</b> – 13 December </p>
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| - | <p><b>• Practice 7:</b> BioBricks™ Assembly</br>
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| - | <p><b>• Practice 8:</b> Ligation</br>
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| - | <p><br><h2><b>Practices Handbook </b><a href="#" class="btn btn-info"><font color="#fff">Back to top</font></a></h2><br></p>
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| - | <p>As part of the activities of the training workshop that was performed by the iGEM-UANL team to prepare the CIDEB high school students interested in participate in the iGEM HSD a practices handbook was made to the students. The handbook was made with the objective to present to the CIDEB participant students an easy, clear and precise panorama about the principle laboratory techniques that are used in the synthetic biology, useful and needed tools for the development of projects in the iGEM HSD competition. In mentioned handbook a series of laboratory practices was included in order to teach the students topics since the use of the Parts Registry of the MIT and the manipulation of plates with BioBricks™, also the manipulation and assembly of them. Each procedure described in the handbook was made during the training workshop. Support material such as some simple bioinformatics tools was used in order to perform mentioned procedures.</p>
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| - | <a href="https://static.igem.org/mediawiki/2012/c/c7/Manual.jpg" rel="lightbox" title="<b>Lab work</b>">
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| - | <img src="https://static.igem.org/mediawiki/2013/3/3d/2012-11-24_15.23.28.jpg" border="2px" width="600" height="400" alt=" Practices Handbook"></a></div>
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| - | <p>The realization of the handbook had the principal purpose of introduce the CIDEB students in the basic, practice and experimental knowledge of the synthetic biology in order to prepare them to the iGEM HSD competition. Inside of the handbook was included a little section where the participants were able to write the obtained results in each practice and themselves could interpret them using the information studied in the workshop, as well as a section of questions that allowed us as instructors to know their weakness and explain the doubts later. We could observe that the use of the handbook with protocols and the experimental practice suring the training workshop were very useful to the participants, because they could develop the ability of working in the molecular biology and genetic engineering and they learn to take important notes to a efficient work in the laboratory. Notes that showed that they were understanding the calculus of the enzymes in cuts and ligations, the gels results, fragments size expected, notes about the bioinfomatics programs, Internet data or books that helped to the participants to obtain a critical mind with their experiments and achieve to formulate their own conclusions.</p>
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| - | <a href="https://static.igem.org/mediawiki/2012/3/30/CIDEB_Handbook_Preview.pdf" target="_blank"><p><br><h3><b>Click Here for a CIDEB Lab Handbook Preview</b></h3><br></p></a>
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In mid-January 2013, students interested on becoming members of the iGEM team UANL 2013 were invited to a four day introductory workshop with the main objective of training them to develop the basic theoretical knowledge for the competition by introducing them to basic Molecular and Synthetic Biology topics, to the tools of Genetic Engineering and to what the competition consists in.jhgfdsdfghgfdsdfghkjhgfdfghjjhgfrtyuijhgf
Description
Objectives
• To prepare the students from the Genomics Biotechnology Degree that are interested in participate in iGEM in the Colleague Division of mentioned workshop in its version 2013 with introductory topics to molecular biology, engineering, nucleic acids manipulation, biology with data processing and synthetic biology.
• To homogenize the interested students knowledge in an easy, clear and precise panorama about the principal laboratory techniques that are used in synthetic biology in order to make them develop the ability to work in the laboratory.
• To aid the students with the doubts that can be presented during the workshop in order to increase their interest in molecular and synthetic biology.
The workshop took place in the Biology School School at the UANL, three hours per day. The topics were the following:
Day 1 January 16
DNA
What is DNA?
Composition of DNA and its properties
Genomics
What are genes?
Genomics organization
Day 2 January 17
Proteins
Function of proteins
Types of Proteins
Genetics Egineering
Characterization through restriction enzymes
Day 3 January 18
Synthetic Biology
BioBricks™ in the Parts Registry
DNA clonation: How can we obtain multiple copies of a gene?
A detailed view to the BioBricks™
Assembly using Building Standards
Day 4 January 19
Checking of the iGEM projects
Project requirements:
• Development of ideas
• Parts construction
• Human Practices
• Poster
• Wiki
• Presentation
By the end of the workshop, besides of acquiring the basic knowledge required for the competition, the students who come from different generations and disciplines, had the opportunity to interact and meet their team mates, strengthening their relation and making the project an experience they can fully enjoy.
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