Team:UANL Mty-Mexico/Notebook

From 2013.igem.org

(Difference between revisions)
Line 56: Line 56:
</table>
</table>
-
<p>This table works as a reference to the parts that were used in the laboratory and in the diary. We have a short name for each part so for example, if a diary entry says "TetR" we mean the complete part "pLambdaCI + TetR". Another important aspect is that we were working with the plasmids pSB1C3, pSB1A3 and PUC57 so here there is the information about the length of the part and the length in each plasmid. </p>
+
<p>This table works as a reference of the parts that were used in the laboratory and in the diary. We have a short name for each part so for example, if a diary entry says "TetR" we mean the complete part "pLambdaCI + TetR". Another important aspect is that we were working with the plasmids pSB1C3, pSB1A3 and PUC57 so here there is the information about the length of the part and the length in each plasmid. </p>
<p><b>August 7th, 2013</b></p>
<p><b>August 7th, 2013</b></p>
-
<p>This day we did Minipreparation of DNA. The parts that were obtained were
+
<p>This day we did Minipreparation of DNA. The parts that were obtained were the following: pCos R Lac1, pLac R GFP, pTetR R mCherry, pTetR, 3-1E</p>
-
== Headline text ==
+
 
-
the following: pCos R Lac1, pLac R GFP, pTetR R mCherry, pTetR, 3-1E</p>
+
<p><b>August 8th, 2013</b></p>
 +
<p><u>Test of mCherry with Thermo-mixer</u>-<u>Cualitative experiment</u></p>
 +
<p>20 Colonies of the dish with pTet-R-mCherry were planted in 0.5 mL of Agar with Kanamycin in order to observe the red color after several hours at 42ᵒ C</p>
 +
 
 +
<table class="table table-striped">
 +
<tr>
 +
<th><</th>
 +
<th><b>Start</b></th>
 +
<th><b>End</b></th>
 +
<th><b>Hours</b></th>
 +
</tr>
 +
<!-- Favorite-Name-Type-Description-Designer-Length-->
 +
<tr>
 +
<td> pLambdaCI + TetR </td>
 +
<td>TetR</td>
 +
<td>2925</td>
 +
<td>2802 </td>
 +
</tr>
 +
<tr>
 +
<td>pLac + GFP</td>
 +
<td> GFP </td>
 +
<td>3162</td>
 +
<td>3034</td>
 +
</tr>
 +
<tr>
 +
<td>pCons LacI</td>
 +
<td> LacI </td>
 +
<td>3483</td>
 +
<td>3352</td>
 +
</tr>
 +
<tr>
 +
<td>pTetR+mCherry</td>
 +
<td>mCherry</td>
 +
<td>3107</td>
 +
<td>2957</td>
 +
</tr>
 +
<tr>
 +
<td>BBa_K1140005</td>
 +
<td> 3-E1 </td>
 +
<td>2811</td>
 +
<td>---</td>
 +
</tr>
 +
</table>
 +
 
</html>
</html>
{{:Team:UANL_Mty-Mexico/Templates:Footer}}
{{:Team:UANL_Mty-Mexico/Templates:Footer}}

Revision as of 19:50, 27 September 2013

Carousel Template for Bootstrap


logo

Notebook

Reference Data

Part Name Part Short name In pSB1A3 (bp) In pSB1C3 (bp) In PUC57 (bp) Part size (bp)
pLambdaCI + TetR TetR 2925 2802 3478 732
pLac + GFP GFP 3162 3034 3715 964
pCons LacI LacI 3483 3352 4036 1,282
pTetR+mCherry mCherry 3107 2957 3660 887
BBa_K1140005 3-E1 2811 --- --- 935

This table works as a reference of the parts that were used in the laboratory and in the diary. We have a short name for each part so for example, if a diary entry says "TetR" we mean the complete part "pLambdaCI + TetR". Another important aspect is that we were working with the plasmids pSB1C3, pSB1A3 and PUC57 so here there is the information about the length of the part and the length in each plasmid.

August 7th, 2013

This day we did Minipreparation of DNA. The parts that were obtained were the following: pCos R Lac1, pLac R GFP, pTetR R mCherry, pTetR, 3-1E

August 8th, 2013

Test of mCherry with Thermo-mixer-Cualitative experiment

20 Colonies of the dish with pTet-R-mCherry were planted in 0.5 mL of Agar with Kanamycin in order to observe the red color after several hours at 42ᵒ C

< Start End Hours
pLambdaCI + TetR TetR 2925 2802
pLac + GFP GFP 3162 3034
pCons LacI LacI 3483 3352
pTetR+mCherry mCherry 3107 2957
BBa_K1140005 3-E1 2811 ---

Creative Commons License

Retrieved from "http://2013.igem.org/Team:UANL_Mty-Mexico/Notebook"