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Team:UANL Mty-Mexico/Notebook
From 2013.igem.org
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<p>Time 10:25 – 9:15 = 22:50 hrs</p> | <p>Time 10:25 – 9:15 = 22:50 hrs</p> | ||
| + | <p><b>August 12th, 2013</b></p> | ||
| + | <p>The experiment was repeated at 42°C with oxygen. It began at 2:15 pm and ended at 1:20 pm</p> | ||
| + | <center><img src="https://static.igem.org/mediawiki/2013/9/99/GertrudisUANL13.jpg" width="200px" height="200px" class="img-rounded">/center> | ||
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{{:Team:UANL_Mty-Mexico/Templates:Footer}} | {{:Team:UANL_Mty-Mexico/Templates:Footer}} | ||
Revision as of 20:12, 27 September 2013
Notebook
Reference Data
| Part Name | Part Short name | In pSB1A3 (bp) | In pSB1C3 (bp) | In PUC57 (bp) | Part size (bp) |
|---|---|---|---|---|---|
| pLambdaCI + TetR | TetR | 2925 | 2802 | 3478 | 732 |
| pLac + GFP | GFP | 3162 | 3034 | 3715 | 964 |
| pCons LacI | LacI | 3483 | 3352 | 4036 | 1,282 |
| pTetR+mCherry | mCherry | 3107 | 2957 | 3660 | 887 |
| BBa_K1140005 | 3-E1 | 2811 | --- | --- | 935 |
This table works as a reference of the parts that were used in the laboratory and in the diary. We have a short name for each part so for example, if a diary entry says "TetR" we mean the complete part "pLambdaCI + TetR". Another important aspect is that we were working with the plasmids pSB1C3, pSB1A3 and PUC57 so here there is the information about the length of the part and the length in each plasmid.
August 7th, 2013
This day we did Minipreparation of DNA. The parts that were obtained were the following: pCos R Lac1, pLac R GFP, pTetR R mCherry, pTetR, 3-1E
August 8th, 2013
Test of mCherry with Thermo-mixer-Cualitative experiment
20 Colonies of the dish with pTet-R-mCherry were planted in 0.5 mL of Agar with Kanamycin in order to observe the red color after several hours at 42ᵒ C
| Start | End | Hours | |
|---|---|---|---|
| 37ºC | 2:50 pm | 7:02 pm | 4 hr 12 min |
| 42ºC | 7:05 pm | 10:35 am | 15 hr 40 min |
Most tubes shown development seeing them on the light.
August 10th, 2013
20 tubes with mCherry were put in the shaker with 2 controls with CDS (with oxygen everyone).
Temperature: 37ᵒ
Revolutions: 900 rpm
Start: 11:35 am -> 9:00 am (Time 21: 35 hours)
The tubes show mCherry expression, some more intense than others.The experiment was repeated at 30°C OVERNIGHT with a Volume of 500ul at 30°C with tubes of 1.5ml. They were left at 4°C meanwhile they were incubated.
| Set | Temperature | Velocity | Time | Live |
|---|---|---|---|---|
| Black Set | 42ºC | 900rpm | 15 hours | :( |
| Blue Set | 37ºC | 900rpm | 21 hours | :) |
| Red Set | 32ºC | 900rpm | :) |
Time 10:25 – 9:15 = 22:50 hrs
August 12th, 2013
The experiment was repeated at 42°C with oxygen. It began at 2:15 pm and ended at 1:20 pm
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