Team:SJTU-BioX-Shanghai/Modeling/Metabolize optimization

From 2013.igem.org

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We call three main reactions which we controlled reaction A, B and C.
We call three main reactions which we controlled reaction A, B and C.
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=Models and Explains=
=Models and Explains=

Revision as of 01:24, 28 September 2013

Metabolismt Optimization

We plan to choose the Fatty acid metabolic pathway for our system's application. We combine three key enzymes in Fatty acid metabolic pathway with our light controlled system. They are TesA, FabI and FabZ which affect three very pivotal reaction process. We expect that we can regulate the proportion of three enzymes by three different light-dCas9 systems to control the productive rate of fatty acid. We use the modeling tools to simulate the regulate process to verification our plan.

Circuit

Three main circuit are as follows:

13sjtumzfFatty acid Biosynthetic Pathway s.jpg

13sjtumzf2.png

13sjtumzf3.png

13sjtumzf4.png

Main Reaction

We call three main reactions which we controlled reaction A, B and C.
13sjtumzfF1 medium.jpg

Models and Explains

In order to make the explaination of our model short and sweet, we simplify three main reactions as follows:


A: A-CoA + M-CoA --> B-ACP (FabI & FabZ)
B: B-ACP + nM-CoA --> F-ACP (FabI & FabZ)
C: F-ACP --> Fatty acid (TesA)
For reaction A, it is a double substrates enzymatic reaction, consideration of substrate self-inhibition, we set this reaction model:
REACTION A.jpg





Bio-X Institutions
Shanghai Jiao Tong University
800, Dongchuan Road
200240 Shanghai, China
igemsjtu2013@gmail.com

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