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INITIAL STATES

Is important to obtain the initial concentration of species in our circuit, since these values will can be used as biologically meaningful initial conditions for subsequent simulations. The initial values describe the state of the cells at the start of the simulation, i.e. placing cells into the agar plate coming from overnight liquid cultures. Should be noted that when the receiver cells grow, they don't have any OHHL in their culture and this affects the concentrations of intracellular species whose production depends on it.