Team:ETH Zurich

From 2013.igem.org

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<li><b>Colisweeper</b><br>Colisweeper is an interactive, biological version of the Minesweeper computer game, based on quorum sensing and chromogenic enzymatic reactions.  
<li><b>Colisweeper</b><br>Colisweeper is an interactive, biological version of the Minesweeper computer game, based on quorum sensing and chromogenic enzymatic reactions.  
The goal is to clear an agar “minefield” without detonating mines. Genetically engineered <i>Escherichia coli</i> colonies are used as sender-cells (mines) and receiver-cells (non-mines). Mines secrete the signaling molecule OHHL whereas non-mines process the signal. To distinguish between OHHL-levels, a library of pLuxR promoters with various OHHL sensitivities was created through site-saturation mutagenesis. High-pass filters were constructed to control the expression of different orthogonal hydrolases in non-mines, depending on the number of surrounding mines. Additionally, the mines express their own hydrolase.
The goal is to clear an agar “minefield” without detonating mines. Genetically engineered <i>Escherichia coli</i> colonies are used as sender-cells (mines) and receiver-cells (non-mines). Mines secrete the signaling molecule OHHL whereas non-mines process the signal. To distinguish between OHHL-levels, a library of pLuxR promoters with various OHHL sensitivities was created through site-saturation mutagenesis. High-pass filters were constructed to control the expression of different orthogonal hydrolases in non-mines, depending on the number of surrounding mines. Additionally, the mines express their own hydrolase.
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<li><b>Minesweeper</b>
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<li><b>Gameplay</b><br>To play Colisweeper, the player has to pipette colorless substrates on a colony on the agar minefield. A single move of pipetting would require the player to choose between two colorless substrates. If the multi-susbtrate is added, this will reveal the identity of the colony- as in the number of mines surrounding a non-mine. The single colorless substrate is pipetted onto a colony if the player is certain of a mine colony. Addition of either substrates produces a defined colored product within minutes, allowing identification of the played colony and the number of mines surrounding it.<br>  
<li><b>Gameplay</b><br>To play Colisweeper, the player has to pipette colorless substrates on a colony on the agar minefield. A single move of pipetting would require the player to choose between two colorless substrates. If the multi-susbtrate is added, this will reveal the identity of the colony- as in the number of mines surrounding a non-mine. The single colorless substrate is pipetted onto a colony if the player is certain of a mine colony. Addition of either substrates produces a defined colored product within minutes, allowing identification of the played colony and the number of mines surrounding it.<br>  
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Revision as of 11:45, 3 October 2013

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  • Colisweeper
    Colisweeper is an interactive, biological version of the Minesweeper computer game, based on quorum sensing and chromogenic enzymatic reactions. The goal is to clear an agar “minefield” without detonating mines. Genetically engineered Escherichia coli colonies are used as sender-cells (mines) and receiver-cells (non-mines). Mines secrete the signaling molecule OHHL whereas non-mines process the signal. To distinguish between OHHL-levels, a library of pLuxR promoters with various OHHL sensitivities was created through site-saturation mutagenesis. High-pass filters were constructed to control the expression of different orthogonal hydrolases in non-mines, depending on the number of surrounding mines. Additionally, the mines express their own hydrolase.
  • Minesweeper
  • Gameplay
    To play Colisweeper, the player has to pipette colorless substrates on a colony on the agar minefield. A single move of pipetting would require the player to choose between two colorless substrates. If the multi-susbtrate is added, this will reveal the identity of the colony- as in the number of mines surrounding a non-mine. The single colorless substrate is pipetted onto a colony if the player is certain of a mine colony. Addition of either substrates produces a defined colored product within minutes, allowing identification of the played colony and the number of mines surrounding it.
  • Information Processing
    The non-mine colonies are designed to distinguish between different concentrations of OHHL and translate this information into expression of different sets of hydrolases. They are equipped with mutated LuxR promoters with different OHHL sensitivities which serve as highpass filters. The promoters were created using site-saturation mutagenesis. Through mutation of the LuxR binding sites we were able to tune the promoters to different OHHL affinities.
  • Hydrolase Reactions
    We use a set of orthogonal hydrolases as our reporter system that react within minutes with the added multi-substrate to produce a visible color. The set of hydrolases such as alkaline phosphatase (phoA), β-galactosidase (lacZ), acetylesterase (aes), β-N-Acetylglucosaminidase (nagZ) and β-glucuronidase (gusA) and their respective substrates react to achieve fast and colorful outputs with each color indicative of the next logical move for the player.
  • From Minesweeper to Colisweeper
    Mines secrete the signaling molecule OHHL whereas non-mines process the signal after diffusion of OHHL. High-pass filters were constructed to control the expression of different orthogonal hydrolases in non-mines, depending on the concentration of the OHHL molecules from the surrounding mines. The constant expression of lacZ enables the flagging of both mines and non mine colonies. Additionally, the mines express their own hydrolase.
  • The Model
    As our system is based on sensing the OHHL concentration, the diffusion of the signalling molecule in the mine field is a vital part of the model. We study the diffusion in a hexagonal grid format with mine and non-mine colonies using a spatio-temporal model. The information from the model was used to validate and improve our system.
  • High pass filter
  • Human practice
    Inspired by our Colisweeper project, we analyzed the relationship between synthetic biology and games. For one thing synthetic biology can be used to play common games in a new way, possibly for educational purposes or as a basis for proof-of-principle experiments for new circuits. More recently synthetic biologists also started to use games as a research tool, an innovative approach to make use of crowd-sourcing and distributed computing. We want to find correlations and discuss possible consequences for Synthetic Biology.
  • Team
    We are a team of seven highly motivated Bachelor- and Master Students at ETH Zürich pursuing various fields such as Biotechnology, Biomedical Engineering, Neurobiology and Bioinformatics. The iGEM project is carried out at one of the youngest departments of ETHZ located in Basel-Department of Biosystems Science and Engineering - flourishing in interdisciplinary biological research. If you're around Basel, make sure to visit our team's lab to play the bio-game Colisweeper!


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