Team:ETH Zurich
From 2013.igem.org
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<li><b>Colisweeper</b><br>Colisweeper is an interactive, biological version of the Minesweeper computer game, based on quorum sensing and chromogenic enzymatic reactions. | <li><b>Colisweeper</b><br>Colisweeper is an interactive, biological version of the Minesweeper computer game, based on quorum sensing and chromogenic enzymatic reactions. | ||
- | The goal is to clear an agar “minefield” without detonating mines. Genetically engineered <i>Escherichia coli</i> colonies are used as sender-cells (mines) and receiver-cells (non-mines). Mines secrete the signaling molecule OHHL whereas non-mines process the signal. To distinguish between OHHL-levels, a library of pLuxR promoters with various OHHL sensitivities was created through site-saturation mutagenesis. High-pass filters were constructed to control the expression of different orthogonal hydrolases in non-mines, depending on the number of surrounding mines. Additionally, the mines express their own hydrolase. | + | The goal is to clear an agar “minefield” without detonating mines. Genetically engineered <i>Escherichia coli</i> colonies are used as sender-cells (mines) and receiver-cells (non-mines). Mines secrete the signaling molecule OHHL( analog to AHL :N-acyl homoserine lactone) whereas non-mines process the signal. To distinguish between OHHL-levels, a library of pLuxR promoters with various OHHL sensitivities was created through site-saturation mutagenesis. High-pass filters were constructed to control the expression of different orthogonal hydrolases in non-mines, depending on the number of surrounding mines. Additionally, the mines express their own hydrolase. |
</li> | </li> | ||
<li><b>Minesweeper</b> | <li><b>Minesweeper</b> |
Revision as of 11:53, 3 October 2013