Team:Marburg/Notebook:Primer list

From 2013.igem.org

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Primer list
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Revision as of 13:35, 3 October 2013

Primer list

No Name Sequence Comment
iMR01 ble-for GTTTCTTCGAATTCGCGGCCGCTTCTAGAATGGCCAAGTTGACCAGTGCCGTTC  
iMR02 ble-rev GTTTCTTCCTGCAGCGGCCGCTACTAGTTCAGTCCTGCTCCTCGGCCACGAAG  
iMR03 cat-for GTTTCTTCGAATTCGCGGCCGCTTCTAGAATGGAGAAAAAAATCACTGGATATA  
iMR04 cat-rev GTTTCTTCCTGCAGCGGCCGCTACTAGTTTACGCCCCGCCCTGCCACTCATCGC  
iMR05 P_fcbP-for GTTTCTTCGAATTCGCGGCCGCTTCTAGAATCCGGAGAGATTCAAAATTCAATC  
iMR06 P_fcbP-rev GTTTCTTCCTGCAGCGGCCGCTACTAGTCTTGACATCTGGCAACCGTGAAATA  
iMR07 P_NR-for GTTTCTTCGAATTCGCGGCCGCTTCTAGACATATGCGGAAGTGACTGTAAACGA  
iMR08 P_NR-rev GTTTCTTCCTGCAGCGGCCGCTACTAGTCGTTCGCACAAGTGGTGACTTTGAA  
iMR09 T_fcpA-for GTTTCTTCGAATTCGCGGCCGCTTCTAGACCTTCCTTAAAAATTTAATTTTCAT  
iMR10 T_fcpA-rev GTTTCTTCCTGCAGCGGCCGCTACTAGTCTCGAGAAAAC TCATCCTGTG CCTT  
iMR11 T_NR-for GTTTCTTCGAATTCGCGGCCGCTTCTAGACAATTTACTTTAAGACTAGC  
iMR12 T_NR-rev GTTTCTTCCTGCAGCGGCCGCTACTAGTTCCGGATGCGTTCATTTTAG  
iMR13 Cat-bb-Pvu-For CCAGACCGTTCAGCTAGATATTACG  
iMR14 Cat-bb-Pvu-Rev CGTAATATCTAGCTGAACGGTCTGG  
iMR15 Cat-bb-Eco-For GCTCATCCGGAGTTCCGTATGGC  
iMR16 Cat-bb-Eco-Rev GCCATACGGAACTCCGGATGAGC  
iMR17 PfcpB-Pvu-For CGACGCTCGCTCCAGCTAGGGCTTCCTTAC  
iMR18 PfcpB-Pvu-Rev GTAAGGAAGCCCTAGCTGGAGCGAGCGTCG  
iMR19 PNR-Pst-For TTCCGGCACCTACAGCGAGGAGTTTTG  
iMR20 PNR-Pst-Rev CAAAACTCCTCGCTGTAGGTGCCGGAA  
iMR21 T_fcpa-Xho-For GGATGAGTTTTCTAGAGGCCGGTCTCC  
iMR22 T-fcpa-Xho-Rev GGAGACCGGCCTCTAGAAAACTCATCC  
iMR23 Seq_P_NR_For CGGGGCTGGCTTAACTAT  
iMR24 Seq_P_NR_Rev GTAAATTGAAGCTTGCATGC  
iMR25 Seq_T_NR und P_fcpB_For ACTTGTGCGAACGGAATT  
iMR26 Seq_T_NR und P_fcpB_Rev CACCGGCGAAGTCGTCCT  
iMR27 Seq_Ble_For CTACGACGCTCGCTCCAG  
iMR28 Seq_Ble_Rev GGCATCCATGGTGAAGACGA  
iMR29 Seq_T_fcpA_For AGATCGGCGAGCAGCCGT  
iMR30 Seq_T_fcpA_Rev CCCGCGCGTTGGCCGATT  
iMR31 Cl4mA_l_for GTTTCTTCGAATTCGCGGCCGCTTCTAGAATGGAAACCCCAGCGCAGCTT  
iMR32 Cl4mA_l_rev GTTTCTTCCTGCAGCGGCCGCTACTAGTCTAACACTCTCCACGGTTGAAGCTC  
iMR33 Cl4mA_h_for GTTTCTTCGAATTCGCGGCCGCTTCTAGAATGGAGTTTGGGCTCAGCTGG  
iMR34 Cl4mA_h_rev GTTTCTTCCTGCAGCGGCCGCTACTAGTTCATTTACCCGGAGACAGGGAG  
iMR35 Seq_pJET12_for cggcgtaatacgactcactata  
iMR36 Seq_pJET12_rev acctacaacggttcctgatg  
iMR37 sequencing_forward_primer_(VF2) tgccacctgacgtctaagaa  
iMR38 sequencing_reverse_primer_(VR) attaccgcctttgagtgagc  
iMR39 Seq_cat.int_rev GCTCATGGAAAACGGTGTAA  
iMR40 eGFP_for GTTTCTTCGAATTCGCGGCCGCTTCTAGAATGGTGAGCAAGGGCGAGG  
iMR41 eGFP_rev GTTTCTTCCTGCAGCGGCCGCTACTAGTTTACTTGTACAGCTCGTCC  
iMR42 SPTP1_for GTTTCTTCGAATTCGCGGCCGCTTCTAGAATGAGATCCTTTTGCATCG Signal/Transitpeptid Stroma 1 AtpC
iMR43 SPTP1_rev GTTTCTTCCTGCAGCGGCCGCTACTAGTTCCATCCATGACAATCGTTG Signal/Transitpeptid Stroma 1 AtpC
iMR44 SPTP2_for GTTTCTTCGAATTCGCGGCCGCTTCTAGAATGAAGACTGCTGTCATTGC Signal/Transitpeptid Stroma 2 FCpD
iMR45 SPTP2_rev GTTTCTTCCTGCAGCGGCCGCTACTAGTGGTGGCAACCGAAGTACGGG Signal/Transitpeptid Stroma 2 FCpD
iMR46 SP1_for GTTTCTTCGAATTCGCGGCCGCTTCTAGAATGACTGCAATAGGGTCTTT Signalpeptid ER 1 PDI
iMR47 SP1_rev GTTTCTTCCTGCAGCGGCCGCTACTAGTGGCAAGACACACTGCTGTG Signalpeptid ER 1 PDI
iMR48 SP2_for GTTTCTTCGAATTCGCGGCCGCTTCTAGAATGCGCTCGTTTCTACTTTG Signalpeptid ER 2 CA-VI
iMR49 SP2_rev GTTTCTTCCTGCAGCGGCCGCTACTAGTAGCAGTCGCGAACGAAGCG Signalpeptid ER 2 CA-VI
iMR50 RFP_for GTTTCTTCGAATTCGCGGCCGCTTCTAGAatggcttcctccgaagacg  
iMR51 RFP_rev ATATGGATCCagcaccggtggagtgacg  
iMR52 MTS_for ATATGGATCCGCGCCGCAGCGGGAGGAAG  
iMR53 MTS_rev GTTTCTTCCTGCAGCGGCCGCTACTAGTCTACCTTTCTAGGAAAAGTTGGCGC  
iMR54 RBS_for aaagaggagaaa Colonie-PCR PlacI-RBS

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