Circuit containing hydrolases

A seven-species model was implemented to model the spatio-temporal behaviour of our multicellular sender–receiver system. The model was based on partial differential equations with Hill functions that captured the activation of protein synthesis as a function of the concentration of the signalling molecule.

For the agar plate and mine cells modules, we use the system of equations and parameters set of the previous simulation.

Mine Cells

The PDEs for the states involved in the sender module are given below:

Equation system 1: System of Differential equations for mine cells.

Agar Plate

The PDE for OHHL in the agar plate is given below:

Equation 2: Processes taking place on the agar plate: diffusion and decay of OHHL

Receiver Cells

Receivers are engineered to respond differently to two OHHL concentration levels. Basically, cells should be capable of produce a visible response and in a reasonable amount of time for the player, in order to be able to discriminate between the presence of 0, 1 or 2 adjacent mines. To achieve this goal, we incorporate two enzymatic reporters (GusA and AES) instead of GFP, under the control of pLux</sub> promoters with different sensitivities. Such enzymes can catalyze the hydrolysis of various chromogenic compounds to give rise to a relatively quick coloured response.

The intracellular species of interest in the receiver cells module include: LuxR, OHHL, LuxR/OHHL complex (denoted as R) and the hydrolases (GusA and AES).

Figure 4: Differential equations of the receiver cells

In addition to new proteins incorporated to the circuit, it is important to emphasize that the grid was changed to a three neighbours setup.

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