Project Overview

Why CRISPRi?

• Regulate genomic genes without changing their promoters.

• "Totipotent". Change the target gene as you want by making some minor changes in gRNA sequence.

Why Light?

• Easy to adjust. Get rid of those laborious work of adding inducing chemicals.

• Accurate.

• Uniformly distribute in cell culture.

Why CRISPRi AND Light?

• Universal. Regulate multiple genes in one same metabolic pathway simultaneously, independently and quantitatively.

• Extensible. The number of genes being regulated can be easily increased if other light sensing systems become available, which will further increase the complexity and accuracy of our regulating system.

Why Computer?

Computer and Electronics! Experience the information century!

• Input your desired expression level into the computer. And just leave the rest to our program and illuminating device to adjust to your specific requirement.