Revision as of 21:09, 18 October 2013

Overview Project Overview

Luminous System Design Device Control

Light Sensors Tri-light! Red Green Blue

Regulator-CRISPRi Introdution to CRISPRi Why CRISPRi? Integrating CRISPRi

Project Overview

"Totipotent"! Change the target gene as you want by making some minor changes in gRNA sequence.

Easy to adjust! Get rid of those laborious work of adding inducing chemicals! :)

Multi-control! lights of different colors can be exerted simultaneously. And one sensor will only be activated by photons with a narrow range of wavelengths.

Computer and Electronics! Experience the information century!

Input your desired expression level into the computer. And just leave the rest to our program and illuminating device to adjust to your specific requirement.

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 =<font color=white>Why CRISPRi AND Light?</font>=
-*Universal. Regulate multiple genes in one same metabolic pathway simultaneously, independently and quantitatively.
-*Extensible. The number of genes being regulated can be easily increased if other light sensing systems become available, which will further increase the complexity and accuracy of our regulating system.
+* Sensors alone? CANNOT be easily applied on genomic genes.
+* CRISPRi alone? HARD to be simultaneous; NOT convenient.
 =<font color=white>Why Computer?</font>=