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The sunlight-driven microalgae ''Phaeodactylum tricornutum'' has been used for the production of complex proteins. A major - however yet unchallenged - advantage of the algae is its ability to secrete proteins directly into the medium. This feature would greatly simplify purification of recombinant proteins, and lower production costs! Here we take advantage of the excellent secretion abilities of ''P. tricornutum'', and make this organism accessible to synthetic biology and iGEM competition ([https://2013.igem.org/Team:Marburg/Project PHAECTORY]). | The sunlight-driven microalgae ''Phaeodactylum tricornutum'' has been used for the production of complex proteins. A major - however yet unchallenged - advantage of the algae is its ability to secrete proteins directly into the medium. This feature would greatly simplify purification of recombinant proteins, and lower production costs! Here we take advantage of the excellent secretion abilities of ''P. tricornutum'', and make this organism accessible to synthetic biology and iGEM competition ([https://2013.igem.org/Team:Marburg/Project PHAECTORY]). | ||
| - | Furthermore, we | + | Furthermore, we use a light-inducible promoter which we have quantitatively characterized to control protein expression in PHAECTORY ([https://2013.igem.org/Team:Marburg/Project:lightcontrol Light control]). Also, we introduced a transplantable element to the registry, which allows autonomous targeting of BioBricks to cell membranes ([https://2013.igem.org/Team:Marburg/Project:RFP Improve a Brick]). We hope you enjoy reading about our projects! |
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Revision as of 10:54, 27 October 2013
The iGEM Marburg proudly presents: PHAECTORY
The use of proteins in medical treatment and diagnosis is steadily increasing. Many of these proteins (e.g. antibodies) have a complex biological structure, which complicates their production. Also, these proteins need to be highly pure. Therefore, a major challenge is the development of systems that produce complex proteins with high purity - best at low costs!The sunlight-driven microalgae Phaeodactylum tricornutum has been used for the production of complex proteins. A major - however yet unchallenged - advantage of the algae is its ability to secrete proteins directly into the medium. This feature would greatly simplify purification of recombinant proteins, and lower production costs! Here we take advantage of the excellent secretion abilities of P. tricornutum, and make this organism accessible to synthetic biology and iGEM competition (PHAECTORY).
Furthermore, we use a light-inducible promoter which we have quantitatively characterized to control protein expression in PHAECTORY (Light control). Also, we introduced a transplantable element to the registry, which allows autonomous targeting of BioBricks to cell membranes (Improve a Brick). We hope you enjoy reading about our projects!
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