• 4 ml cell culture was harvested.
• Pellet was dissolved in 1 mL PBS.
• 0.5 ml glass beads were added.
• Tubes were shaken for 3 min → color shift from brown to green.
• TCA precipitation was performed.

• 4 ml cell culture was harvested.
• Dilution in 300 µL 8M urea buffer.
• Shock freezing of suspension by means of liquid nitrogen.
• Two steel balls were added → Tube was shock freezed again.
• Cell disruption was carried out by 3 times shaking for 30 sec and another shock freezing.

• 4 ml cell culture was harvested (5 min centrifugation at full speed).
• The Supernatent was discarded and the pellet frozen in liquid nitrogen.
• The frozen pellet was resuspended in 0.68 ml IP buffer with 3.4 µl PIC and sonicated (48 cycles, 30 sec on, 30 sec off, 4°C, high).
• Extract was centrifuged at 7000 rcf for 5 min, the supernatant was transferred in a new reaction tube.
• 1,7 µl supernatant of cell lysis were mixed with 350 µl 70 % TCA and incubated for 20 min on ice.
• The mixture was centrifuged for 15 min at 20.000 rcf and the pellet washed twice with 1 ml acetone.
• The pellet was dried at RT and resuspended in 60 µl 8M ureabuffer (15 min 60 °C).

10 ml of cultures grown under different light conditions (green, blue, red, full light spectrum and darkness) were harvested in 15 ml Falcon-tubes (5 min centrifugation at full speed). Supernatants were discarded and pellets frozen in liquid nitrogen.

Frozen cell-pellets were resuspended in 1,7 ml IP buffer with 8,5 µl PIC and sonicated (48 cycles, 30 sec on, 30 sec off, 4°C, high). Extract was centrifuged at 7000 rcf for 5 min, the supernatant was transferred in a new reaction tube.

1,7 µl supernatant of cell lysis were mixed with 350 µl 70 % TCA and incubated for 20 min on ice. The mixture was centrifuged for 15 min at 20.000 rcf and the pellet washed twice with 1 ml acetone. The pellet was dried at RT and resuspended in 60 µl 8M ureabuffer (15 min 60 °C).

10 µg of each sample were used for SDS-PAGE (Westernblot).