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Team:ETH Zurich/Notebook
From 2013.igem.org
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| - | <div style="font-size:18px;font-family: | + | <div style="font-size:18px;font-family:Verdana;position:static;margin-top:10px"> <b>Kick off event</b> 19.06.13 </div> |
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| - | <div style="font-size:18px;font-family: | + | <div style="font-size:18px;font-family:Verdana;position:static"> <b>Brainstorming</b> 19.06.13 - 3.07.13 </div> |
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| - | <div style="font-size:18px;font-family: | + | <div style="font-size:18px;font-family:Verdana;position:static"> <b>Start Work</b> 4.07.13 </div> |
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| - | <div style="color:white;font-size:18px;font-family: | + | <div style="color:white;font-size:18px;font-family:Verdana;position:static"> <b>Week 1</b> : Planning of responsibilities, start the wiki editing,logo slogan and name for the project, design of experiment, making buffers, media, chemical competent cells, choose the biobricks and do ctransformations of the first bricks. Start modeling. </div> |
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| - | <div style="color:white;font-size:18px;font-family: | + | <div style="color:white;font-size:18px;font-family:Verdana;position:static"> <b>Week 2</b> : Transformation of all the bricks and clonning to built our pathways. Decide about 4 hydrolases : NagZ, PhoA, GusA and Aes and the according substrates to color them. We encouter some difficulties during transformiation of triple knockout cells with the hydrolases</div> |
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| - | <div style="color:white;font-size:18px;font-family: | + | <div style="color:white;font-size:18px;font-family:Verdana;position:static"> <b>Week 3</b> : All transformation and clonning is done.</div> |
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| - | <div style="color:white;font-size:18px;font-family: | + | <div style="color:white;font-size:18px;font-family:Verdana;position:static"> <b>Week 4</b> :Start of AHL diffusion experiments for characterization of the AHL diffusion. The receiver cells with GFP are done and work well on plates an liquid culture. Primer design for the mutation of LuxR promoter to alter sensitvity.</div> |
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| - | <div style="color:white;font-size:18px;font-family: | + | <div style="color:white;font-size:18px;font-family:Verdana;position:static"> <b>Week 5</b> : Working on AHL diffusion, transform an other strain with T7 polymerase to test the substrates. Working on transformation of the triple knockout cells. Retransform LuxI construct because the first transformation din't have an RBS.</div> |
Revision as of 13:25, 18 August 2013
Kick off event 19.06.13
Brainstorming 19.06.13 - 3.07.13
Start Work 4.07.13
Week 1 : Planning of responsibilities, start the wiki editing,logo slogan and name for the project, design of experiment, making buffers, media, chemical competent cells, choose the biobricks and do ctransformations of the first bricks. Start modeling.
Week 2 : Transformation of all the bricks and clonning to built our pathways. Decide about 4 hydrolases : NagZ, PhoA, GusA and Aes and the according substrates to color them. We encouter some difficulties during transformiation of triple knockout cells with the hydrolases
Week 3 : All transformation and clonning is done.
Week 4 :Start of AHL diffusion experiments for characterization of the AHL diffusion. The receiver cells with GFP are done and work well on plates an liquid culture. Primer design for the mutation of LuxR promoter to alter sensitvity.
Week 5 : Working on AHL diffusion, transform an other strain with T7 polymerase to test the substrates. Working on transformation of the triple knockout cells. Retransform LuxI construct because the first transformation din't have an RBS.
