Goals
A dark box can provide a sample with a 3-color luminous source.
The light intensity should be equal in samples.
The device should be easy to be assembled and operated.
The device should have expansibility.
Design
The luminous device is a bridge connecting electric parts and biological parts. In our project, we chose flasks as our sample container, LEDs as our luminous sources. Since the distribution of light intensity is not evenly in entire space, we must choose a suitable light direction. Side and top were finally abandoned because the walls of flasks can reflect light so that the light intensity is not even in samples.
After deciding the direction, the distance between bottom and luminous sources should be decided. The distribution of light intensity close to LEDs is not even, in another word, the range of the intensity is fairly broad. However, the intensity will be evenly distributed when a longer distance between light source and the sample. After some basic test, we found that the distribution is mainly even if the distance reaches about 15cm.
The dark box contains two parts, the upper part holds a flask and the lower part is empty, which make sure that the distance between sample and light source is 15cm. At the bottom of darkbox lies a micro circuit board which contains some electric circuit components and three LEDs: red, green and blue. The LEDs can be controled by MCU(Microprogrammed Control Unit) via lines.
LED board
LED board contains basic circuit components which play the terminal of control system. The board is so tiny and easy to make that is has high expansibility.
 Fig 2 circuit diagram of LED board
There are three kinds of circuit components on the board: adjustable resistors, audions and LEDs.
Adjustable resistors are used to keep the initial light intensity same; audions are used to amplify the signal delivered from MCUs.
To make the boards easy to be installed, we put LEDs on one side and other components on the other side. Thus we can fix them at the bottom of dark boxes.
MCU part
MCU part translates the digital value into electronic signal. It has one COM port as input port and 8 PWM(Pulse Width Modulation) output port, each of them can adjust light intensity of one LED, so one board can control two 3-color LED boards.
One MCU can not meet our need, because two parallel experiments can show the differents among three genetic factors. In ideal situation, we need nine 3-color LED boards work at the same time. In other word, we need four MCU boards, and we finally use two as prototype.
Due to our modular design, the expansibility is fairly great, the LED boards can be changed with 1-color or 2-color boards, and the number of terimals doesn't have any limit with enough MCUs.
Next:[http://igem.bio-x.cn/Team:SJTU-BioX-Shanghai/Project/Luminous_device/Control Control]
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