Team:Colombia Uniandes/Parts

From 2013.igem.org

Revision as of 02:31, 28 September 2013 by Alejandro.otero.b (Talk | contribs)

Parts

Glucocorticoid sensor

Glucocorticoid sensor

Here are the parts sent to the registry:

PartsGC.jpg

You can see the confirmation gels of the parts below:




PCR confirmation: E1GF-E1GF(2)-E2GF-E2GF(2)-WM-E3GF-E3GF(2)-E4GF-E4GF(2)
Co-transformation: WM-E1GF2-E2GF-E3GF1-E3GF2-E4GF-WM- E3GF3-E4GF2-E2GF2
PCR confirmation of the parts cloned in the pSB1C3 backbone using the specifically designed primers 15 and 31 (see primers)


Also, the fluorescence assays for the E*GF parts

Unsaturated curve where we see how the fluorescence increases after the addition of dexamethasone, a glucocorticoid
Saturated curve for fluorescence. We can see the different strengths depending on the number of UAS boxes


Nickel Removal System

Nickel Removal System

Here are the parts sent to the registry:

PartsNi.jpg
Prcna-HoxN and HoxN-RFP


Retrieved from "http://2013.igem.org/Team:Colombia_Uniandes/Parts"