INITIAL STATES

Is important to obtain the steady state concentration of some of the species in our circuit, since these values can be used as biologically meaningful initial conditions for subsequent simulations. When cells are placed on the agar plate, they come from an overnight liquid culture, thus we are assuming that the cells have reached the steady state. Should be noted that when the receiver cells grow, there is not OHHL in the medium and this affects the concentrations of intracellular species whose production depends on it.

Initial state for the Mine Cells

Figure 1: Steady state concentrations of Mine Cell species.