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Team:ETH Zurich/Modeling/Overview
From 2013.igem.org
The digital bacterial-based minesweeper
We created a 2D spatio-temporal model of the Colisweeper bacterial game to evaluate our network, validate the design and get some insights of how we can improve our system. Essentially, we studied the diffusion of OHHL in a hexagonal grid setup with mine and non-mine colonies. For the simulation, we used COMSOL Multiphysics. Most of the model parameters were derived from literature that was supported with experimental evidence, and very few of the parameters were fitted. It needs to be mentioned that there was a close interplay with the model and experiments in our project which facilitated the refining of four system. Based on this premise we generate data from experiments to validate the model, but also use to model to predict the behavior of our system and help further experimental designs.
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OHHL Diffusion concentration gradient after 24 hours.
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Level of GFP expression, after 11 hours of incubation, depending on the number of mine cells surrounding a colony. On the left is shown the experimental result (fluorescence) and on the right the simulation results are displayed, corresponding to concentration of GFP (mol/m3). Mine colonies are delimited in green.
Note: For parameter values and references click on the parameter of interest or have a look at the parameters section.
