Team:Colombia Uniandes/Journal

Contents 1 Dear Journal! :) 1.1 24-Jun-2013(AllJournals!) 1.2 25-Jun-2013(AllJournals!) 1.3 26-Jun-2013(AllJournals!)

Dear Journal! :)

Here we will place all the information about the work we have been doing during this time, it will be named by date and project, for example: 14-Mar-2013(NickelJournal) or 14-Mar-2013(GlucoJournal). We hope you enjoy our work and thoughts as much as we do!

24-Jun-2013(AllJournals!)

After we went over the project, we proceed to design our primers so we could place our order, taking into account that we are going to use Fusion PCR as our main protocol. Our primers take around 2 weeks to arrive… sooo meanwhilee all the team started making our E.coli babies electrocompetent, so we can work with them later on.

25-Jun-2013(AllJournals!)

So today we took our bacterial cells (Top 10) onto an LB medium (no antibiotics). We let it ON at 37°C on the shaker. Also we prepared all the material we need for tomorrow: ddwater, 10% glycerol, LB medium and microfuge tubes.

26-Jun-2013(AllJournals!)

Today we are going to start preparing our cells, we inoculated in the morning and placed all our material that needs to be cold in the fridge 4°C(ddWater and glycerol), also the centifuge rotor needs to be chilled. Then we just waited for the perfect OD and made our cells around 4:00pm. We made a lot!

We placed them in the -80, ready to start our work!!!