Team:Colombia Uniandes/Parts

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Parts

Glucocorticoid sensor

Glucocorticoid sensor

Here are the parts sent to the registry:

PartsGC.jpg

You can see the confirmation gels of the parts below:



PCR confirmation: E1GF-E1GF(2)-E2GF-E2GF(2)-WM-E3GF-E3GF(2)-E4GF-E4GF(2)
Co-transformation: WM-E1GF2-E2GF-E3GF1-E3GF2-E4GF-WM- E3GF3-E4GF2-E2GF2


Also, the fluorescence assays for the E*GF parts

Unsaturated curve where we see how the fluorescence increases after the addition of dexamethasone, a glucocorticoid
Saturated curve for fluorescence. We can see the different strengths depending on the number of UAS boxes


Nickel Removal System

Nickel Removal System

Here are the parts sent to the registry:

PartsNi.jpg
Prcna-HoxN and HoxN-RFP


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