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2 additional sequence samples were sent out for sequencing.
The samples were incubated for 1 h at 37° C.
Gel substances
Expactations
After that, a gel electrophoresis was made, but failed and has to be repeated the next day
4 colonies of pSB1A3-iBB496315 were inoculated in 5 ml LB medium containing ampicillin.
Obviously the wrong plasmid was sent out for sequencing. Due to the lack of enough plasmids for an additional sequence analysis, we will redo a new miniprep.
The chemo competent E. coli DH5α cells were transformed with the plasmids pSB1A3-iBB4+iBB10+iBB96315 and pSB1A3-iBB4+iBB13+iBB96315 and plated on LB-Amp-plates. The plates were incubated over night at 37° C.
The plasmids were isolated with the “QIAprep Spin Miniprep Kit” (Qiagen, Düsseldorf) according to the producer instructions.
Worked as expected.
All plasmids are brought to Marian who will transform them in P. tricornutum cells.
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