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From 2013.igem.org
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- 17:14, 11 September 2013 (diff | hist) N 10 September 2013 (Created page with "<div>Today we transformed our aptamers from SELEX 12 using both TOPO TA cloning and pGEM vector cloning (See protocols for details).</div> <div>In addition, we repeted colony PCR...") (top)
- 17:06, 11 September 2013 (diff | hist) N 9 September 2013 (Created page with "<div>Today we did colony pcr of streptavidin colony, using vr2 and vf2 primers.</div> <div>We have run gel for sel 11 and 12, double bands were there so we did gel extraction.</d...") (top)
- 20:31, 6 September 2013 (diff | hist) Team:NU Kazakhstan/Protocols
- 20:10, 6 September 2013 (diff | hist) N 6 September 2013 (Created page with "<div>DOT ELISA (see protocols for details) was done to see the binding between anti-CEA antibodies and biotynilated CEA. The results showed very slight binding at very high conce...") (top)
- 20:04, 6 September 2013 (diff | hist) Team:NU Kazakhstan/Protocols
- 20:03, 6 September 2013 (diff | hist) Team:NU Kazakhstan/Protocols
- 18:06, 5 September 2013 (diff | hist) N 4 September 2013 (Created page with "BL21 competent cells were transformed with plasmids containing RBS, RFP, GFP, and YFP from the kit. Cells were plated on LB agar plates containing chloramphinecol. They were left...") (top)
- 18:03, 5 September 2013 (diff | hist) N 5 September 2013 (Created page with "<div>DOT ELISA for biotynilated CEA was performed.</div> <div>The growth of colonies of BL21 transformed cells containing plasmids with RBS, YFP, and RFP were observed. Colonies ...") (top)
- 17:56, 5 September 2013 (diff | hist) 2 September 2013 (top)
- 17:55, 5 September 2013 (diff | hist) 2 September 2013
- 02:24, 2 September 2013 (diff | hist) Team:NU Kazakhstan/Modeling
- 17:34, 25 August 2013 (diff | hist) Team:NU Kazakhstan/Protocols
- 17:32, 25 August 2013 (diff | hist) Team:NU Kazakhstan/Attributions
- 17:31, 25 August 2013 (diff | hist) Team:NU Kazakhstan/Human practices
- 17:31, 25 August 2013 (diff | hist) Team:NU Kazakhstan/Safety
- 17:30, 25 August 2013 (diff | hist) Team:NU Kazakhstan/Notebook
- 17:30, 25 August 2013 (diff | hist) Team:NU Kazakhstan/Aptamers
- 17:29, 25 August 2013 (diff | hist) Team:NU Kazakhstan/Yeast
- 17:28, 25 August 2013 (diff | hist) Team:NU Kazakhstan/E.Coli
- 17:28, 25 August 2013 (diff | hist) Team:NU Kazakhstan/Modeling
- 17:27, 25 August 2013 (diff | hist) Team:NU Kazakhstan/Team
- 17:26, 25 August 2013 (diff | hist) Team:NU Kazakhstan
- 17:25, 25 August 2013 (diff | hist) Team:NU Kazakhstan/Protocols
- 18:21, 24 August 2013 (diff | hist) N 24 August 2013 (Created page with "<div>1. SDS PAGE now was placed in destaining solution again overnight.</div> <div>2. Red colonies of cells, transformed with plasmid containing RFP, have grown on LB agar and ch...") (top)
- 18:16, 24 August 2013 (diff | hist) N 23 August 2013 (Created page with "<div>1. SDS PAGE for biotinylated CEA protein was done (see protocols for the details) and the gel was left in coomassie blue staining solution overnight.</div> <div>2. BL21 comp...") (top)
- 16:57, 19 August 2013 (diff | hist) N 19 August 2013 (Created page with "<div>1. No colonies grew on yesterday plates with antibiotics. It may mean that transformation did not occur.</div> <div>2. Very weak colonies have grown on control LB agar plate...") (top)
- 15:57, 19 August 2013 (diff | hist) N 18 August 2013 (Created page with "TOPO TA cloning (see protocols for the procedure) was done with BL 21 competent cells, and the cells were plated and left overnight at 37degC.") (top)
- 18:12, 18 August 2013 (diff | hist) Team:NU Kazakhstan/Protocols
- 17:58, 18 August 2013 (diff | hist) Team:NU Kazakhstan/Protocols
- 17:54, 18 August 2013 (diff | hist) Team:NU Kazakhstan/Protocols
- 17:22, 18 August 2013 (diff | hist) Team:NU Kazakhstan/Protocols
- 15:22, 17 August 2013 (diff | hist) Team:NU Kazakhstan/Team
- 15:21, 17 August 2013 (diff | hist) Team:NU Kazakhstan/Aptamers
- 15:21, 17 August 2013 (diff | hist) Team:NU Kazakhstan/Yeast
- 15:20, 17 August 2013 (diff | hist) Team:NU Kazakhstan/E.Coli
- 15:20, 17 August 2013 (diff | hist) Team:NU Kazakhstan/Modeling
- 15:19, 17 August 2013 (diff | hist) Team:NU Kazakhstan/Notebook
- 15:19, 17 August 2013 (diff | hist) Team:NU Kazakhstan/Protocols
- 15:18, 17 August 2013 (diff | hist) Team:NU Kazakhstan/Safety
- 15:18, 17 August 2013 (diff | hist) Team:NU Kazakhstan/Human practices
- 15:17, 17 August 2013 (diff | hist) Team:NU Kazakhstan/Attributions
- 15:17, 17 August 2013 (diff | hist) Team:NU Kazakhstan
- 15:15, 17 August 2013 (diff | hist) N Team:NU Kazakhstan/Human practices (Created page with "<html> <img src="http://s11.postimg.org/yo0r6q8c3/logon.jpg" width="900" height="200"></html> <html> </body> <head> <title>NU_Kazakhstan</title> <style type="text/css"> ul { ...")
- 15:15, 17 August 2013 (diff | hist) Team:NU Kazakhstan/Safety
- 15:14, 17 August 2013 (diff | hist) N Team:NU Kazakhstan/Aptamers (Created page with "<html> <img src="http://s11.postimg.org/yo0r6q8c3/logon.jpg" width="900" height="200"></html> <html> </body> <head> <title>NU_Kazakhstan</title> <style type="text/css"> ul { ...")
- 15:14, 17 August 2013 (diff | hist) N Team:NU Kazakhstan/Yeast (Created page with "<html> <img src="http://s11.postimg.org/yo0r6q8c3/logon.jpg" width="900" height="200"></html> <html> </body> <head> <title>NU_Kazakhstan</title> <style type="text/css"> ul { ...")
- 15:14, 17 August 2013 (diff | hist) N Team:NU Kazakhstan/E.Coli (Created page with "<html> <img src="http://s11.postimg.org/yo0r6q8c3/logon.jpg" width="900" height="200"></html> <html> </body> <head> <title>NU_Kazakhstan</title> <style type="text/css"> ul { ...")
- 15:13, 17 August 2013 (diff | hist) Team:NU Kazakhstan/Modeling
- 15:13, 17 August 2013 (diff | hist) N Team:NU Kazakhstan/Protocols (Created page with "<html> <img src="http://s11.postimg.org/yo0r6q8c3/logon.jpg" width="900" height="200"></html> <html> </body> <head> <title>NU_Kazakhstan</title> <style type="text/css"> ul { ...")
- 15:12, 17 August 2013 (diff | hist) Team:NU Kazakhstan/Notebook
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