What about those hydrolases ?

We use hydrolases to realize a colorimetric response by conversion of a specific substrate. We will use five hydrolases : Beta-Galactosidase (lacZ),Beta-Glucuronidase (GusA); alkaline phosphatase (phoA);Glycoside hydrolase (NagZ) and the acetyl esterase (aes).

On this page you will find all relevant information on the hydrolases and enzyme substrate reactions based on papers and our results.

Beta-galactosidase(LacZ)

Colorimetric response in liquid culture

Colorimetric response on LB-Agar

Fig.5: X-Gal on LacZ expressing colony

Fig.6: Beta-green-X-gal on LacZ expressing colony

Alkaline phosphatase(PhoA)

Colorimetric response in liquid culture

Colorimetric response on LB-Agar

Fig.2: NPP on PhoA expressing colony

Acetyl esterase(Aes)

Colorimetric response in liquid culture

Colorimetric response on LB-Agar

Fig.1: Magenta butyrate on Aes expressing colony

Beta-glucuronidase(GusA)

Colorimetric response in liquid culture

Colorimetric response on LB-Agar

Fig.3 Magenta glucuronide on GusA expressing colony

β-N-Acetylglucosaminidase(NagZ)

Colorimetric response in liquid culture

Colorimetric response on LB-Agar