What about those hydrolases ?

We use hydrolases to realize a colorimetric response by conversion of a specific substrate. We will use five hydrolases : Beta-Galactosidase (lacZ),Beta-Glucuronidase (GusA); alkaline phosphatase (phoA);Glycoside hydrolase (NagZ) and the acetyl esterase (aes).

On this page you will find all relevant information on the hydrolases and enzyme substrate reactions based on papers and our results.

Beta-galactosidase(LacZ)

Chemical conversion



Colorimetric response in liquid culture

Colorimetric response on LB-Agar

Fig.5: X-Gal on LacZ expressing colony

Fig.6: Beta-green-X-gal on LacZ expressing colony

Alkaline phosphatase(PhoA)

Chemical conversion



Colorimetric response in liquid culture

Colorimetric response on LB-Agar

Fig.2: NPP on PhoA expressing colony

Acetyl esterase(Aes)

Chemical conversion



Colorimetric response in liquid culture

Colorimetric response on LB-Agar

Fig.1: Magenta butyrate on Aes expressing colony

Beta-glucuronidase(GusA)

Chemical conversion



Colorimetric response in liquid culture

Colorimetric response on LB-Agar

Fig.3 Magenta glucuronide on GusA expressing colony

β-N-Acetylglucosaminidase(NagZ)

Chemical conversion



Colorimetric response in liquid culture

Colorimetric response on LB-Agar