Notebook : July 9

Summary :

For regulation system :

• extraction of BioBrick BBa_K1155000 in concentrated medium for further DNA sequencing.
• the terminator BBa_B0010 in PSB1A2 plasmid was transformed into competent cells and then was cultured on solid medium
• the plasmid PSB3K3 was transformed into competent cells and then was cultured on solid medium for confirmation

For sensor system:

• A series of digestion, ligation were performed for BioBrick BphR2, BphR1, BphA1

Lab work

constructing